评估糖尿病 C57BL/6 小鼠精子染色质/DNA 完整性、形态和 Catsper 表达。

Evaluation of sperm chromatin/DNA integrity, morphology, and Catsper expression on diabetic C57BL/6 mice.

机构信息

Department of Anatomical Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.

出版信息

Cell Mol Biol (Noisy-le-grand). 2022 Feb 28;68(2):8-18. doi: 10.14715/cmb/2022.68.2.2.

DOI:10.14715/cmb/2022.68.2.2

PMID:35869727

Abstract

BACKGROUND

Diabetes is associated with reproductive impairment on the male reproductive system and causes complications such as decreased libido, fertility, spermatogenesis, sperm motility, and morphology. High levels of blood sugar may affect sperm quality and reduce the potential for male fertility. Increased levels of sperm DNA damage is often associated with reduced count and motility or abnormal morphology.

METHODS

This experimental study was conducted in Mashhad University of Medical Sciences. In this work, 40 mice (C57BL/6) were divided randomly into 4 groups: 1) Control, 2) Diabetic, 3) Diabetic + Insulin, and 4) Sham. After 35 days, the right epididymis of all specimens was used for Real-Time PCR and left epididymis for evaluation of sperm parameters using Aniline blue, Toluidine blue, Papanicolaou, and immunohistochemical study. Also, testes were applied for immunohistochemical, TUNEL studies, and biochemical assay.

RESULTS

Results of this study showed that chromatin integrity, morphology, cation channels of sperm (Catsper) expression, and biochemical factors level were significantly changed in diabetic mice in comparison to other groups (P<0.05) and treatment with insulin improved these parameters.

CONCLUSION

Our findings showed that the sperm parameters such as DNA integrity, morphology, and Catsper expression change in diabetic mice.

摘要

背景

糖尿病与男性生殖系统的生殖损伤有关，并导致性欲降低、生育能力下降、精子发生、精子活力和形态异常等并发症。高血糖水平可能会影响精子质量，降低男性生育能力。精子 DNA 损伤水平升高通常与精子数量和活力降低或形态异常有关。

方法

这项实验性研究是在马什哈德医科大学进行的。在这项工作中，将 40 只小鼠（C57BL/6）随机分为 4 组：1）对照组，2）糖尿病组，3）糖尿病+胰岛素组，和 4）假手术组。35 天后，所有标本的右侧附睾用于实时 PCR，左侧附睾用于评价精子参数，包括苯胺蓝、甲苯胺蓝、巴氏染色和免疫组织化学研究。此外，还对睾丸进行了免疫组织化学、TUNEL 研究和生化分析。