College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
College of Enology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Environ Res. 2022 Nov;214(Pt 2):113946. doi: 10.1016/j.envres.2022.113946. Epub 2022 Jul 21.
This study developed a closed-circuit biorefinery process for full conversion of lignocellulose into ethanol, biogas and organic fertilizer with zero waste on a pilot scale. In the process, subcritical water pretreatment could effectively break the structure of wheat straw (WS), and ethanol was obtained from pretreated wheat straw (PWS) using two batches of simultaneous saccharification and fermentation (SSF). The pretreatment and ethanol fermentation wastes were reused for biogas and organic fertilizer production by anaerobic digestion (AD), whereas the pretreatment and ethanol conversion efficiency were reduced when supernatant after AD was recovered for next batch pretreatment. The yields of ethanol (0.08-0.09 g/g), biogas (0.05-0.10 L/g) and organic fertilizer (0.55-0.79 g/g) were demonstrated through mass balance. Furthermore, the hidden problems were exposed on pilot-scale conversion process, and several strategies were provided for optimizing the biorefinery process in the future.
本研究开发了一种闭路生物炼制工艺，可在中试规模上将木质纤维素完全转化为乙醇、沼气和有机肥，实现零废物排放。在该过程中，亚临界水预处理可有效破坏小麦秸秆（WS）的结构，然后采用两批次同步糖化发酵（SSF）从预处理后的小麦秸秆（PWS）中获得乙醇。预处理和乙醇发酵废物通过厌氧消化（AD）再用于沼气和有机肥生产，而当 AD 后的上清液回收用于下一批次预处理时，预处理和乙醇转化效率会降低。通过质量平衡，证明了乙醇（0.08-0.09 g/g）、沼气（0.05-0.10 L/g）和有机肥（0.55-0.79 g/g）的产率。此外，还揭示了中试规模转化过程中的潜在问题，并为未来优化生物炼制工艺提供了几种策略。
