Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Bioresour Technol. 2015 Jan;175:17-22. doi: 10.1016/j.biortech.2014.10.060. Epub 2014 Oct 18.
The production of ethanol from wheat straw (WS) by dilute acid pretreatment, bioabatement of fermentation inhibitors by a fungal strain, and simultaneous saccharification and fermentation (SSF) of the bio-abated WS to ethanol using an ethanologenic recombinant bacterium was studied at a pilot scale without sterilization. WS (124.2g/L) was pretreated with dilute H2SO4 in two parallel tube reactors at 160°C. The inhibitors were bio-abated by growing the fungus aerobically. The maximum ethanol produced by SSF of the bio-abated WS by the recombinant Escherichia coli FBR5 at pH 6.0 and 35°C was 36.0g/L in 83h with a productivity of 0.43gL(-1)h(-1). This value corresponds to an ethanol yield of 0.29g/g of WS which is 86% of the theoretical ethanol yield from WS. This is the first report on the production of ethanol by the recombinant bacterium from a lignocellulosic biomass at a pilot scale.
从小麦秸秆（WS）中通过稀酸预处理生产乙醇，用真菌菌株生物降解发酵抑制剂，以及使用产乙醇重组菌对生物降解的 WS 进行同时糖化和发酵（SSF）生产乙醇，在无需灭菌的情况下进行了中试规模的研究。WS（124.2g/L）在两个平行管反应器中于 160°C 下用稀 H2SO4 预处理。通过有氧培养真菌来生物降解抑制剂。在 pH 值为 6.0 和 35°C 下，重组大肠杆菌 FBR5 对生物降解的 WS 进行 SSF，最大乙醇产量为 83h 时的 36.0g/L，生产速率为 0.43gL（-1）h（-1）。这一值相当于 WS 的 0.29g/g 的乙醇得率，为 WS 理论乙醇得率的 86%。这是首次在中试规模上用重组菌从木质纤维素生物质生产乙醇的报道。
