Trigueros Esther, Ramos Cipriano, Alonso-Riaño Patricia, Beltrán Sagrario, Sanz María Teresa
Department of Biotechnology and Food Science, Chemical Engineering Division, University of Burgos, Plza. Misael Bañuelos s/n, Burgos 09001, Spain.
Ind Eng Chem Res. 2023 Feb 16;62(8):3503-3514. doi: 10.1021/acs.iecr.2c04132. eCollection 2023 Mar 1.
The feasibility of industrial subcritical water treatment on residue through scaling up from the lab to pilot system in discontinuous mode (geometric scale-up factor = 50), at 130 and 175 °C (5% biomass), was investigated. The maximum volumes of the reactors were 500 mL at the lab-scale and 5 L at the pilot-scale system. At 175 °C, faster extraction/hydrolysis was observed for the pilot plant, but maximum yields were similar: 71.4 and 78.6% for galactans, 9.8 and 10.4% for glucans, and 92.7 and 86.1% for arabinans in pilot scale and lab scale, respectively, while the yields for proteins accounted nearly 40%. The highest yields for amino acids were observed for the smallest ones, while lower yields were determined for polar amino acids. The total phenolic content and color intensity progressively increased along time at lab scale, while a plateau was reached at the pilot level. Lower extraction yields but reproducible results were obtained at 130 °C. Finally, the pilot scale was essayed at a higher biomass loading (15%), and successful results were obtained, supporting the feasibility of the scaling-up process.
研究了在130和175℃（生物质含量5%）下，通过从实验室规模到中试系统的非连续模式放大（几何放大系数 = 50）对残渣进行工业亚临界水处理的可行性。实验室规模反应器的最大体积为500 mL，中试规模系统的最大体积为5 L。在175℃时，中试装置的提取/水解速度更快，但最大产率相似：中试规模和实验室规模的半乳聚糖产率分别为71.4%和78.6%，葡聚糖产率分别为9.8%和10.4%，阿拉伯聚糖产率分别为92.7%和86.1%，而蛋白质产率接近40%。最小的氨基酸产率最高，而极性氨基酸的产率较低。在实验室规模下，总酚含量和颜色强度随时间逐渐增加，而在中试水平达到平稳状态。在130℃时获得的提取产率较低，但结果具有可重复性。最后，在更高的生物质负载量（15%）下对中试规模进行了试验，并取得了成功结果，支持了放大过程的可行性。
