Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, FL, USA.
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL, USA.
Sci Rep. 2022 Jul 23;12(1):12599. doi: 10.1038/s41598-022-15562-3.
Oncolytic viral therapy is a recent advance in cancer treatment, demonstrating promise as a primary treatment option. To date, the secondary metabolic effects of viral infection in cancer cells has not been extensively studied. In this work, we have analyzed early-stage metabolic changes in cancer cells associated with oncolytic myxoma virus infection. Using GC-MS based metabolomics, we characterized the myxoma virus infection induced metabolic changes in three cancer cell lines-small cell (H446) and non-small cell (A549) lung cancers, and glioblastoma (SFxL). We show that even at an early stage (6 and 12 h) myxoma infection causes profound changes in cancer cell metabolism spanning several important pathways such as the citric acid cycle, fatty acid metabolism, and amino acid metabolism. In general, the metabolic effects of viral infection across cell lines are not conserved. However, we have identified several candidate metabolites that can potentially serve as biomarkers for monitoring oncolytic viral action in general.
溶瘤病毒治疗是癌症治疗的最新进展，有望成为主要治疗选择。迄今为止，病毒感染对癌细胞的次级代谢影响尚未得到广泛研究。在这项工作中，我们分析了与溶瘤性兔病毒性出血症病毒感染相关的癌细胞早期代谢变化。使用基于 GC-MS 的代谢组学，我们对三种癌细胞系（小细胞肺癌 H446 和非小细胞肺癌 A549 以及神经胶质瘤 SFxL）中溶瘤性兔病毒性出血症病毒感染诱导的代谢变化进行了表征。我们表明，即使在早期阶段（6 和 12 小时），兔病毒性出血症感染也会导致跨越柠檬酸循环、脂肪酸代谢和氨基酸代谢等几个重要途径的癌细胞代谢发生深刻变化。一般来说，病毒感染对细胞系的代谢影响并不保守。然而，我们已经确定了一些候选代谢物，它们可能作为监测溶瘤病毒作用的生物标志物。
