College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong, China.
Qingdao Vocational and Technical College of Hotel Management, Qingdao, 266003, Shandong, China.
Appl Microbiol Biotechnol. 2022 Sep;106(17):5615-5628. doi: 10.1007/s00253-022-12082-y. Epub 2022 Jul 25.
Vibrio parahaemolyticus is a common pathogen in aquatic products, such as shellfishes. Laboratory-based simulated studies demonstrated that V. parahaemolyticus can tolerate high hydrostatic pressure (HHP) up to 20 MPa. However, the molecular mechanisms of high-pressure adaptation remain unclear. Herein, we analyzed the physiological changes and transcriptomic responses of V. parahaemolyticus ATCC 17,802 under HHP conditions to determine the possible survival mechanisms. Under HHP conditions, the morphology of V. parahaemolyticus was notably changed exhibiting the coccoid microbial cells. The transcriptome analysis revealed that there were 795 differentially expressed genes (DEGs) under the 20 MPa condition, including 406 upregulated DEGs and 389 downregulated DEGs. Most of the downregulated DEGs encoded proteins related to energy metabolism, such as citrate synthase (gltA), pyruvate kinase (pyk), and glyceraldehyde-3-phosphate dehydrogenase (gapA). Many of the upregulated DEGs encoded proteins related to adhesion and virulence factors, such as RNA polymerase σ factor (rpoE), L-threonine 3-dehydrogenase, and bacterial nucleotide signal c-di-GMP (WU75_RS02745 and WU75_RS07185). In our proposed mechanism model, V. parahaemolyticus responds to HHP stress through RNA polymerase σ factor RpoE. These findings indicate that V. parahaemolyticus cells may adopt a complex adaptation strategy to cope with HHP stress. KEY POINTS: •The transcriptomic response of Vibrio parahaemolyticus under HHP conditions was studied for the first time. •V. parahaemolyticus may adopt a complex adaptation strategy to cope with HHP stress. •ToxRS and RpoE played an important role in sensing and responding the HHP signal.
副溶血性弧菌是水产品（如贝类）中的一种常见病原体。基于实验室的模拟研究表明，副溶血性弧菌可以耐受高达 20 MPa 的高静压（HHP）。然而，其高压适应的分子机制尚不清楚。在此，我们分析了副溶血性弧菌 ATCC 17802 在 HHP 条件下的生理变化和转录组响应，以确定可能的生存机制。在 HHP 条件下，副溶血性弧菌的形态明显发生变化，呈现出球菌样微生物细胞。转录组分析显示，在 20 MPa 条件下有 795 个差异表达基因（DEGs），包括 406 个上调 DEGs 和 389 个下调 DEGs。大多数下调的 DEGs 编码与能量代谢相关的蛋白质，如柠檬酸合酶（gltA）、丙酮酸激酶（pyk）和甘油醛-3-磷酸脱氢酶（gapA）。许多上调的 DEGs 编码与粘附和毒力因子相关的蛋白质，如 RNA 聚合酶σ因子（rpoE）、L-苏氨酸 3-脱氢酶和细菌核苷酸信号 c-di-GMP（WU75_RS02745 和 WU75_RS07185）。在我们提出的机制模型中，副溶血性弧菌通过 RNA 聚合酶σ因子 RpoE 对 HHP 应激做出反应。这些发现表明，副溶血性弧菌细胞可能采用复杂的适应策略来应对 HHP 应激。关键点：• 首次研究了副溶血性弧菌在 HHP 条件下的转录组响应。• 副溶血性弧菌可能采用复杂的适应策略来应对 HHP 应激。• ToxRS 和 RpoE 在感知和响应 HHP 信号方面发挥着重要作用。
