Kadi Hamza, Tanriverdi Cayci Yeliz, Yener Nazik, Gur Vural Demet, Bilgin Kemal, Birinci Asuman
Department of Virology Laboratory, Samsun Veterinary Control Institute, Samsun, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
Indian J Med Microbiol. 2022 Oct-Dec;40(4):557-559. doi: 10.1016/j.ijmmb.2022.07.004. Epub 2022 Jul 22.
Elizabethkingia anophelis was firstly isolated from the midgut of the Anopheles gambiae mosquito in 2011. After this year, it was isolated in some intensive care cases in Africa and Asia. This study, it was aimed to confirm the identification of E. anophelis in the blood of a pediatric patient.
After the suspicious bacteria were grown on blood agar, MALDI-TOF MS and 16s rRNA gene sequencing methods were used to identify and an antibiotic susceptibility test was carried out by Vitek 2 Compact system according to the EUCAST. Finally, a phylogenetic tree was created based on the 16s rRNA gene region.
The isolate was identified as E. anophelis by both methods. It was found to be resistant to all beta-lactam antibiotics and also susceptible to ciprofloxacin and levofloxacin. According to the 16S rRNA-based phylogenetic tree, our isolate clustered within a branch containing other E. anophelis.
These findings will guide clinicians in choosing which antibiotic to choose if they encounter this agent. Also, the clinicians should be vigilant against this agent, as it is a newly emerging infectious agent in Turkey.
嗜蚊伊氏菌于2011年首次从冈比亚按蚊的中肠中分离出来。此后，在非洲和亚洲的一些重症监护病例中也分离出了该菌。本研究旨在确认一名儿科患者血液中嗜蚊伊氏菌的鉴定结果。
将可疑细菌接种在血琼脂上培养后，采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和16S rRNA基因测序方法进行鉴定，并根据欧洲抗菌药物敏感性试验委员会（EUCAST）的标准，使用Vitek 2 Compact系统进行药敏试验。最后，基于16S rRNA基因区域构建系统发育树。
两种方法均将分离菌株鉴定为嗜蚊伊氏菌。发现该菌对所有β-内酰胺类抗生素耐药，但对环丙沙星和左氧氟沙星敏感。根据基于16S rRNA的系统发育树，我们的分离菌株聚集在包含其他嗜蚊伊氏菌的一个分支内。
这些发现将指导临床医生在遇到该病原体时选择合适的抗生素。此外，临床医生应警惕该病原体，因为它是土耳其一种新出现的感染病原体。
