School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, People's Republic of China; College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, People's Republic of China.
School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, People's Republic of China; State Key Laboratory of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, People's Republic of China.
Life Sci. 2022 Oct 1;306:120828. doi: 10.1016/j.lfs.2022.120828. Epub 2022 Jul 21.
Oxidative stress is an important part of the development of NAFLD, and hepatic injury can be prevented by inhibiting oxidative stress. In this study, we investigated the potential role of sinapine in protecting the liver. LPS was selected to establish the oxidative stress model of THLE-2 cells, and the treatment concentrations of LPS (5 μg/mL) and sinapine (5 μM, 20 μM, and 80 μM) were determined by toxicity experiments. The MDA of the sinapine (80 μM) pretreatment group was 1.09 ± 0.13 nmol/mg prot which was reduced by 27.67 % compared with the LPS group. Furthermore, SOD and GSH-Px levels were significantly increased by 40.61 % and 49.60 %, respectively. And the ROS levels of 20 and 80 μM sinapine were reduced by 31.47 % and 40.31 %, respectively (p < 0.01) compared with the model group. The mitochondrial membrane potential had similar results. It was also found that sinapine can significantly down-regulate the level of MCJ protein (p < 0.01), which is related to oxidative stress. Our results indicate that sinapine can maintain liver health by down-regulating the expression of MCJ protein to inhibit oxidative stress, which provides a theoretical basis for the use of sinapine as an inhibitor of MCJ.
氧化应激是 NAFLD 发展的重要组成部分，抑制氧化应激可以预防肝损伤。在本研究中，我们研究了芥子碱抑制 MCJ 蛋白表达从而抑制氧化应激对肝脏的保护作用。采用 LPS 建立 THLE-2 细胞氧化应激模型，通过毒性实验确定 LPS（5μg/ml）和芥子碱（5μM、20μM 和 80μM）的处理浓度。芥子碱（80μM）预处理组的 MDA 为 1.09±0.13nmol/mg prot，比 LPS 组降低了 27.67%。此外，SOD 和 GSH-Px 水平分别显著升高了 40.61%和 49.60%。20μM 和 80μM 芥子碱组的 ROS 水平分别降低了 31.47%和 40.31%（p<0.01）。线粒体膜电位也有类似的结果。还发现芥子碱可以显著下调与氧化应激相关的 MCJ 蛋白水平（p<0.01）。我们的研究结果表明，芥子碱可以通过下调 MCJ 蛋白的表达来抑制氧化应激，从而维持肝脏健康，为芥子碱作为 MCJ 抑制剂的应用提供了理论依据。
