Li Youdong, Duan Mengxin, Liu Guoyan, Liang Li, Liu Xiaofang, Zhang Jixian, Wen Chaoting, Xu Xin
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
School of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China.
Foods. 2024 Oct 11;13(20):3237. doi: 10.3390/foods13203237.
Sinapine is a natural polyphenol from the cruciferous plant family that has anti-aging effects but is low in bioavailability. To improve the bioavailability and therapeutic effect of sinapine, sinapine-crosslinked dual-protein-based hydrogels were prepared using soy protein isolate as a cross-linking agent. The preparation conditions were optimized by single-factor experiments, and the optimal ratios were obtained as follows: the concentration of sinapine was 300 μg/mL; the water-oil ratio was 1:3. The encapsulation rate was greater than 95%, and the drug loading capacity was 3.5 mg/g. In vitro, digestion experiments showed that the dual-protein-based hydrogels as a drug carrier stabilized the release of sinapine and improved the bioavailability of sinapine by 19.3%. The IC50 of DPPH antioxidants was 25 μg/mL as determined by in vitro digestion, and the antioxidant capacity of ABTS was about 20% higher than that of glutaraldehyde control. This is due to the addition of sinapine to enhance the antioxidant properties of the system. It can be seen that the developed hydrogels have potential applications in related fields, such as food nutrition fortification and drug delivery.
芥子碱是一种来自十字花科植物家族的天然多酚，具有抗衰老作用，但生物利用度较低。为了提高芥子碱的生物利用度和治疗效果，以大豆分离蛋白为交联剂制备了芥子碱交联的双蛋白基水凝胶。通过单因素实验优化了制备条件，得到的最佳比例如下：芥子碱浓度为300μg/mL；水油比为1:3。包封率大于95%，载药量为3.5mg/g。体外消化实验表明，双蛋白基水凝胶作为药物载体可稳定芥子碱的释放，并使芥子碱的生物利用度提高19.3%。通过体外消化测定，DPPH抗氧化剂的IC50为25μg/mL，ABTS的抗氧化能力比戊二醛对照高约20%。这是由于添加了芥子碱增强了体系的抗氧化性能。可见，所制备的水凝胶在食品营养强化和药物递送等相关领域具有潜在应用价值。
