Briggs J A, Montiel M M, Briggs R C, Hnilica L S
Mol Cell Biochem. 1987 Mar;74(1):29-41. doi: 10.1007/BF00221910.
Chicken erythroid nuclei were prepared using four published methods. Our findings indicate that nuclei prepared by nitrogen cavitation are less likely to be contaminated with plasma membrane fragments than those made by procedures involving cell disruption by hypotonic lysis. However, globin gene sequences were much less sensitive to DNase I digestion in nuclei prepared by nitrogen cavitation. This suggests that the conformation of chromatin was altered by the cavitation procedure. Analysis of the proteins solubilized during limited DNase I digestion of nuclei prepared by both hypotonic lysis and cavitation revealed no appreciable differences in HMG proteins but a notable difference in the RNP-associated proteins and core histones.
鸡红细胞核采用四种已发表的方法制备。我们的研究结果表明，与通过低渗裂解进行细胞破碎的方法制备的细胞核相比，通过氮气空化制备的细胞核受质膜碎片污染的可能性更小。然而，在通过氮气空化制备的细胞核中，珠蛋白基因序列对DNase I消化的敏感性要低得多。这表明空化过程改变了染色质的构象。对通过低渗裂解和空化制备的细胞核进行有限DNase I消化过程中溶解的蛋白质分析表明，高迁移率族（HMG）蛋白没有明显差异，但核糖核蛋白相关蛋白和核心组蛋白存在显著差异。
