从聚丙烯酰胺凝胶转移至硝酸纤维素膜上的组织特异性核抗原的鉴定
Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.
作者信息
Glass W F, Briggs R C, Hnilica L S
出版信息
Science. 1981 Jan 2;211(4477):70-2. doi: 10.1126/science.7003713.
PMID:7003713
Abstract
Nonhistone protein antigens resolved by electrophoresis in sodium dodecyl sulfate were identified immunochemically after being transferred to nitrocellulose. Use of antiserum to dehistonized chromatin from Novikoff hepatoma revealed numerous protein antigens specific to the chromatin of Novikoff hepatoma in comparison to that of normal rat liver.
摘要
在十二烷基硫酸钠中通过电泳分离出的非组蛋白抗原，转移至硝酸纤维素膜后进行免疫化学鉴定。使用针对诺维科夫肝癌去组蛋白染色质的抗血清，与正常大鼠肝脏相比，发现了许多诺维科夫肝癌染色质特有的蛋白质抗原。
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