经脱氧核糖核酸酶II消化后，从鸡红细胞核中释放出富含珠蛋白基因的染色质组分。

Release of a globin gene enriched chromatin fraction from chicken erythrocyte nuclei following DNase II digestion.

作者信息

Goldsmith M E

出版信息

Nucleic Acids Res. 1981 Dec 11;9(23):6471-85. doi: 10.1093/nar/9.23.6471.

DOI:10.1093/nar/9.23.6471

PMID:6275364

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327617/

Abstract

Mild digestion of chicken erythrocyte nuclei with deoxyribonuclease II results in the release of a chromatin fraction which is 4- to 13-fold enriched for the globin coding sequences when compared to total chicken DNA. The remaining nuclear pellet is depleted in these sequences. A maximum of 25% of the globin genes have been recovered in the released fraction. The addition of 5 mM sodium butyrate to the digestion buffer is required to obtain reproducible globin gene enrichment. The released fraction contains equimolar amounts of the four core histones and a subset of the nonhistone chromosomal proteins. The globin genes are released as large chromatin fragments which exceed the 1.6 kilobase size of the transcribed portion of the gene.

摘要

用脱氧核糖核酸酶II对鸡红细胞核进行轻度消化，会释放出一种染色质组分，与鸡的总DNA相比，该组分中珠蛋白编码序列的富集度提高了4至13倍。剩余的核沉淀中这些序列减少。释放的组分中最多回收了25%的珠蛋白基因。需要在消化缓冲液中添加5 mM丁酸钠才能获得可重复的珠蛋白基因富集。释放的组分含有等摩尔量的四种核心组蛋白和一部分非组蛋白染色体蛋白。珠蛋白基因以超过该基因转录部分1.6千碱基大小的大染色质片段形式释放。