Center for Reproductive Medicine, Shandong University, No. 157 Jingliu Road, Jinan, 250021 Shandong, China.
Comput Math Methods Med. 2022 Jul 14;2022:7933166. doi: 10.1155/2022/7933166. eCollection 2022.
The study was aimed at investigating the influence of human chorionic gonadotropin (hCG) hormone on oocyte maturation in the patients with polycystic ovary syndrome (PCOS). A total of 54 patients with PCOS who received maturation (IVM) treatment in the Cheeloo College of Medicine, Shandong University, were divided into two groups: one group who underwent hCG injections was the observation group (OG; = 27) and other was the control group (CG; = 27) with no hCG injection. The oocyte development and the expression of steroid hormone synthesis-related genes including gonadotropin-releasing hormone receptor (GnRHR), Conexin43, epidermal growth factor-related genes, luteinizing hormone/choriogonadotropin receptor (LHCGR), epiregulin (EREG), and vascular endothelial growth factor (VEGF) were examined. The human ovarian granulosa cell line (SVOG cells) and ovarian epithelial cell line (HOSEpiC cells) were employed to analyze the effect of hCG on the biological behaviour of cells. As a result, OG showed higher normal fertilization, cleavage, and high-qualified embryo rate than CG. Expression levels of GnRHR, Cx43, LHCGR, EREG, and VEGF were significantly elevated in granulosa cells in the OG group. Western blot revealed that phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and rapamycin (mTOR) proteins were decreased in granulosa cells under hCG intervention. A biological behaviour test indicated that the multiplication capacity of hCG-intervened SVOG and HOSEpiC was increased, while the apoptosis was decreased. In conclusion, hCG could accelerate follicular development and oocyte maturation by activating oocyte maturation genes in PCOS patients, which could significantly improve and popularize the application of IVM technology.
本研究旨在探讨人绒毛膜促性腺激素（hCG）对多囊卵巢综合征（PCOS）患者卵母细胞成熟的影响。共有 54 名在山东大学齐鲁医学院接受卵母细胞成熟（IVM）治疗的 PCOS 患者被分为两组：一组接受 hCG 注射，为观察组（OG；n=27），另一组未接受 hCG 注射，为对照组（CG；n=27）。检测了卵母细胞发育和与甾体激素合成相关基因的表达，包括促性腺激素释放激素受体（GnRHR）、连接蛋白 43（Conexin43）、表皮生长因子相关基因、促黄体生成素/绒毛膜促性腺激素受体（LHCGR）、表皮调节素（EREG）和血管内皮生长因子（VEGF）。用人卵巢颗粒细胞系（SVOG 细胞）和卵巢上皮细胞系（HOSEpiC 细胞）分析 hCG 对细胞生物学行为的影响。结果显示，OG 的正常受精率、卵裂率和优质胚胎率均高于 CG。OG 组颗粒细胞中 GnRHR、Cx43、LHCGR、EREG 和 VEGF 的表达水平显著升高。Western blot 显示，颗粒细胞中磷酸肌醇 3-激酶（PI3K）、蛋白激酶 B（AKT）和雷帕霉素（mTOR）蛋白减少。生物学行为试验表明，hCG 干预的 SVOG 和 HOSEpiC 的增殖能力增加，凋亡减少。总之，hCG 可通过激活 PCOS 患者卵母细胞成熟基因加速卵泡发育和卵母细胞成熟，显著提高和推广 IVM 技术的应用。
