Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province 266000, China.
Comput Math Methods Med. 2022 Jul 14;2022:7416572. doi: 10.1155/2022/7416572. eCollection 2022.
To probe into the impact of atorvastatin on RANKL expression in rats during the retention stage after orthodontic tooth movement and its associated molecular mechanisms.
After establishing an orthodontic tooth movement model, the left teeth of the retention-stage rats were the maintained side, and the right teeth were the nonmaintained side, which were given physiological saline or atorvastatin dosing at 7d, 14d, and 21d, respectively, by tube feeding, in order to keep the rats as a control group at the beginning of the retention stage. A model of the rat's upper jaw gypsum in each group was made at various time points to measure the distance at which the teeth relapsed. The pathological slices of the upper jaw arch were taken separately for TRAP staining observation.
Compared to the physiological saline group, the recurrence distance of rats in the atorvastatin group was visually lower ( < 0.05), and the number of bone-breaking cells was signally lower ( < 0.05); P-5b, PTH, VitD3, GC, IL-1, and IL-17 expressions ( < 0.05) were visually decreased, while IL-11 expression was elevated ( < 0.05).
The atorvastatin given to rats during the retention stage after orthodontic tooth movement inhibits RANKL expression and may function through OPG/RANKL/RANK system.
探讨阿托伐他汀在正畸牙移动保持期对大鼠 RANKL 表达的影响及其相关分子机制。
建立正畸牙移动模型后，将保持期大鼠的左侧牙齿作为维持侧，右侧牙齿作为非维持侧，分别于第 7、14、21 天经管饲给予生理盐水或阿托伐他汀，使大鼠在保持期开始时作为对照组。在各时间点分别制作各组大鼠上颌石膏模型，测量牙齿复发距离。分别取上颌弓病理切片进行 TRAP 染色观察。
与生理盐水组相比，阿托伐他汀组大鼠的复发距离肉眼更低（<0.05），破骨细胞数量信号更低（<0.05）；PTH、VitD3、GC、IL-1、IL-17 表达（<0.05）肉眼下降，而 IL-11 表达升高（<0.05）。
在正畸牙移动保持期给予大鼠阿托伐他汀可抑制 RANKL 表达，可能通过 OPG/RANKL/RANK 系统发挥作用。
