Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Oral Dis. 2020 Mar;26(2):370-380. doi: 10.1111/odi.13239. Epub 2019 Dec 6.
The present study was designed to investigate the effects of microRNA-21 (miR-21) on orthodontic tooth movement.
The orthodontic tooth movement model was established in C57BL/6 and miR-21 mice with or without implantation of activated T cells. Histological and histomorphometrical analyses were performed by hematoxylin-eosin staining. Tartrate-resistant acid phosphate staining was used to analyze the osteoclast numbers during tooth movement. Enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and immunohistochemistry analysis were used to examine the expression of RANKL and OPG.
In miR-21 mice, the distance of tooth movement was retarded, the osteoclast number was decreased, and serum RANKL expression was strongly reduced. MiR-21 promoted the secretion of RANKL from activated T cells. Furthermore, activated T cells could partially rescue the decreased orthodontic tooth movement distance in miR-21 mice. MiR-21 was shown to promote orthodontic tooth movement by modulating the RANKL/OPG balance in T cells.
The impact of miR-21 on tooth movement was better elucidated, furthering our understanding of its role and clinical applications in orthodontics.
本研究旨在探讨 microRNA-21（miR-21）对正畸牙齿移动的影响。
通过在 C57BL/6 和 miR-21 小鼠中植入活化的 T 细胞，建立正畸牙齿移动模型。通过苏木精-伊红染色进行组织学和组织形态计量学分析。使用抗酒石酸酸性磷酸酶染色分析牙齿移动过程中的破骨细胞数量。酶联免疫吸附试验、逆转录聚合酶链反应和免疫组织化学分析用于检测 RANKL 和 OPG 的表达。
在 miR-21 小鼠中，牙齿移动的距离延迟，破骨细胞数量减少，血清 RANKL 表达明显降低。miR-21 促进活化 T 细胞分泌 RANKL。此外，活化的 T 细胞可部分挽救 miR-21 小鼠中减少的正畸牙齿移动距离。miR-21 通过调节 T 细胞中的 RANKL/OPG 平衡来促进正畸牙齿移动。
本研究更好地阐明了 miR-21 对牙齿移动的影响，进一步了解其在正畸学中的作用和临床应用。
