Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, Norway.
Faculty of Medicine, University of Oslo, Oslo, Norway.
Front Endocrinol (Lausanne). 2022 Jul 7;13:874977. doi: 10.3389/fendo.2022.874977. eCollection 2022.
Adipokines are highly active biopeptides involved in glucose metabolism, insulin regulation and the development and progression of obesity and its associated diseases. It includes, among others, adiponectin, visfatin and tumor necrosis factor alpha (TNFα). The sources of adipokines and their associations with glucometabolic variables are not completely understood.
In this cross-sectional study, we aimed to investigate whether gene expression levels in subcutaneous adipose tissue (SAT) of selected adipokines and their corresponding circulating levels associate with the amount of AT in superficial (sSAT), deep (dSAT) and visceral AT (VAT), assessed by computed tomography (CT). Any association with glucometabolic variables were also explored.
In 103 healthy Caucasian men, aged 39.5 years, fasting venous blood and SAT samples from the gluteal region were collected. Ninety-four of the participants underwent CT assessment of the abdominal AT, which was divided into VAT, sSAT and dSAT. Circulating levels of adipokines were measured by ELISA and AT gene-expression by PCR. Insulin sensitivity was determined by glucose clamp, assessing glucose disposal rate (GDR).
Circulating adiponectin and TNFα gene expression correlated inversely and positively to the amount of AT in all three compartments (r=-0.266 to -0.276, p<0.05 for all) and (r=0.323 - 0.368, p<0.05 for all), respectively, with strongest correlations to the amount in sSAT and dSAT. When dividing AT compartments into quartiles, a tendency was observed towards lower circulating adiponectin and higher TNFα gene expression levels, respectively, with increasing amount of sSAT and dSAT. Circulating adiponectin correlated inversely to insulin, C-peptide and waist circumference (r=-456 to -0.373, p<0.001) and positively to GDR (r=0.356, p<0.001). AT-expressed visfatin correlated inversely to insulin and C-peptide (r=-0.370 and r=-0.404, p<0.001).
Increased amount of AT is associated with lower levels of adiponectin and increased levels of TNFα AT expression.
脂肪因子是高度活跃的生物肽，参与葡萄糖代谢、胰岛素调节以及肥胖及其相关疾病的发生和发展。其中包括脂联素、内脏脂肪素和肿瘤坏死因子-α（TNFα）。脂肪因子的来源及其与糖代谢变量的关系尚不完全清楚。
在这项横断面研究中，我们旨在研究选定脂肪因子的皮下脂肪组织（SAT）中的基因表达水平及其相应的循环水平是否与计算机断层扫描（CT）评估的浅表层（sSAT）、深层（dSAT）和内脏脂肪组织（VAT）中的 AT 量相关。还探讨了与糖代谢变量的任何关联。
在 103 名年龄为 39.5 岁的健康白种男性中，采集空腹静脉血和臀区 SAT 样本。94 名参与者接受了腹部 AT 的 CT 评估，将其分为 VAT、sSAT 和 dSAT。通过 ELISA 测量循环脂肪因子水平，通过 PCR 测量 AT 基因表达。通过葡萄糖钳夹测定胰岛素敏感性，评估葡萄糖处置率（GDR）。
循环脂联素和 TNFα 基因表达与所有三个部位的 AT 量呈负相关（r=-0.266 至-0.276，p<0.05 ）和正相关（r=0.323 至 0.368，p<0.05 ），与 sSAT 和 dSAT 的量相关性最强。当将 AT 部位分为四等分时，观察到随着 sSAT 和 dSAT 量的增加，循环脂联素水平降低，TNFα 基因表达水平升高。循环脂联素与胰岛素、C 肽和腰围呈负相关（r=-456 至-0.373，p<0.001），与 GDR 呈正相关（r=0.356，p<0.001）。AT 表达的内脏脂肪素与胰岛素和 C 肽呈负相关（r=-0.370 和 r=-0.404，p<0.001）。
AT 量的增加与脂联素水平降低和 TNFα AT 表达水平升高有关。
