Alves Marco G, Moreira Ângela, Guimarães Marta, Nora Mário, Sousa Mario, Oliveira Pedro F, Monteiro Mariana P
Multidisciplinary Unit for Biomedical Research, UMIB-FCT, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Portugal.
Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Portugal.
BBA Clin. 2017 May 11;8:1-6. doi: 10.1016/j.bbacli.2017.05.001. eCollection 2017 Dec.
Adipose tissue (AT) is involved in dysmetabolism pathogenesis. Regional fat distribution and functioning may contribute to obesity-related metabolic disorders and adverse health outcomes. Specific fat depots are suggested to possess unique biological properties, but specific metabolic profiles of subcutaneous (SAT) and visceral adipose tissue (VAT) remain unknown. We aimed to characterize VAT and SAT glucose metabolism, and their correlation with body mass index (BMI). AT samples from patients (n = 12; F:M, 9:3) with a mean age of 46 years (26-83 years) and an average BMI of 29.6 kg/m (18-37 kg/m) were used. VAT and SAT explants were obtained during elective laparoscopy, either cholecystectomy for uncomplicated cholelithiasis or gastric bypass for severe obesity. Explants were placed in insulin-free cell culture media and their metabolic profile was established by proton nuclear magnetic resonance. AT explants display a glucose and pyruvate consumption and acetate production that is region-dependent according to the patients BMI. In VAT, glucose consumption was positively correlated with BMI, while alanine and lactate production were negatively correlated with BMI, whereas in SAT the patients BMI did not affect AT secretome suggesting that increased BMI promotes a metabolic reprogramming of VAT towards de novo lipogenesis. This region-dependent metabolic reprogramming of AT associated with BMI was autonomous of insulin. This data, although preliminary, suggests that there is a BMI-related remodeling of glucose metabolism in VAT. Targeting this BMI-induced metabolic shift may represent a potential target to counteract unwanted consequences derived from visceral adiposity.
脂肪组织（AT）参与代谢紊乱的发病机制。局部脂肪分布和功能可能导致与肥胖相关的代谢紊乱和不良健康后果。特定的脂肪储存部位被认为具有独特的生物学特性，但皮下脂肪组织（SAT）和内脏脂肪组织（VAT）的具体代谢特征仍不清楚。我们旨在表征VAT和SAT的葡萄糖代谢及其与体重指数（BMI）的相关性。使用了平均年龄为46岁（26 - 83岁）、平均BMI为29.6 kg/m²（18 - 37 kg/m²）的患者（n = 12；女性:男性，9:3）的AT样本。VAT和SAT外植体是在择期腹腔镜手术期间获取的，要么是因单纯性胆结石进行胆囊切除术，要么是因严重肥胖进行胃旁路手术。将外植体置于无胰岛素的细胞培养基中，并通过质子核磁共振确定其代谢特征。根据患者的BMI，AT外植体显示出葡萄糖和丙酮酸消耗以及乙酸盐产生存在区域依赖性。在VAT中，葡萄糖消耗与BMI呈正相关，而丙氨酸和乳酸产生与BMI呈负相关，而在SAT中，患者的BMI不影响AT分泌组，这表明BMI增加促进了VAT向从头脂肪生成的代谢重编程。这种与BMI相关的AT区域依赖性代谢重编程不依赖于胰岛素。这些数据虽然是初步的，但表明VAT中存在与BMI相关的葡萄糖代谢重塑。针对这种由BMI诱导的代谢转变可能代表了一个潜在靶点，以对抗内脏肥胖带来的不良后果。
