Rattanaumpa Tidapa, Maensiri Santi, Ngamchuea Kamonwad
School of Chemistry, Institute of Science, Suranaree University of Technology 111 University Avenue, Suranaree, Muang Nakhon Ratchasima 30000 Thailand
School of Physics, Institute of Science, Suranaree University of Technology 111 University Avenue, Suranaree, Muang Nakhon Ratchasima 30000 Thailand.
RSC Adv. 2022 Jun 27;12(29):18709-18721. doi: 10.1039/d2ra03126d. eCollection 2022 Jun 22.
Herein, we demonstrate the superior electrocatalytic activities of microporous carbon in the oxidation of three molecular biomarkers, ascorbic acid (AA), dopamine (DA), and uric acid (UA), which are co-present in biological fluids. The voltammetric responses of AA, DA, and UA at the low-cost microporous carbon electrode show significantly better sensitivity and selectivity than other more expensive and commonly used electrode materials such as copper(ii) oxide, copper(i) oxide, and carbon nanotube. Differential pulse voltammetry at the microporous carbon electrode allows the detection of AA, DA, and UA with linear ranges of 100-2000 μM (AA), 10-150 μM (DA), and 10-150 μM (UA), sensitivities of 6.8 ± 0.2 nA μM (AA), 261.4 ± 3.4 nA μM (DA), and 93.5 ± 2.0 nA μM (UA), and detection limits of 23.1 μM (AA), 0.2 μM (DA), and 1.7 μM (UA). The method has been validated with a synthetic urine sample to yield ∼100% recoveries for all three analytes. The developed method has been further applied in the investigation of the peroxide scavenging activity of UA.
在此，我们展示了微孔碳在三种分子生物标志物（抗坏血酸（AA）、多巴胺（DA）和尿酸（UA））氧化反应中的卓越电催化活性，这些生物标志物共存于生物流体中。在低成本的微孔碳电极上，AA、DA和UA的伏安响应显示出比其他更昂贵且常用的电极材料（如氧化铜（II）、氧化亚铜（I）和碳纳米管）显著更好的灵敏度和选择性。在微孔碳电极上进行差分脉冲伏安法可检测AA、DA和UA，其线性范围分别为100 - 2000 μM（AA）、10 - 150 μM（DA）和10 - 150 μM（UA），灵敏度分别为6.8 ± 0.2 nA μM（AA）、261.4 ± 3.4 nA μM（DA）和93.5 ± 2.0 nA μM（UA），检测限分别为23.1 μM（AA）、0.2 μM（DA）和1.7 μM（UA）。该方法已通过合成尿液样本验证，对所有三种分析物的回收率约为100%。所开发的方法已进一步应用于研究UA的过氧化物清除活性。
