Wei Xiaowei, Cao Jing, Lan Haiyan
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.
Front Genet. 2022 Jul 7;13:927830. doi: 10.3389/fgene.2022.927830. eCollection 2022.
Basic helix-loop-helix (bHLH) transcription factors play important roles in plant growth, development, metabolism, hormone signaling pathways, and responses to abiotic stresses. However, comprehensive genomic and functional analyses of genes have not yet been reported in desert euhalophytes. , an annual C halophyte without Kranz anatomy, presents high photosynthetic efficiency in harsh natural habitats and is an ideal plant for identifying transcription factors involved in stress resistance. In this study, 83 genes in were identified and categorized into 21 subfamilies based on conserved motifs, gene structures, and phylogenetic analysis. Functional annotation enrichment revealed that the majority of SabHLHs were enriched in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in the response to stress conditions, as transcription factors. A number of -acting elements related to plant hormones and stress responses were also predicted in the promoter regions of , which were confirmed by expression analysis under various abiotic stress conditions (NaCl, mannitol, low temperature, ABA, GA, MeJA, and SA); most were involved in tolerance to drought and salinity. SabHLH169 (076) protein localized in the nucleus was involved in transcriptional activity, and gene expression could be affected by different light qualities. This study is the first comprehensive analysis of the gene family in These data will facilitate further characterization of their molecular functions in the adaptation of desert plants to abiotic stress.
基本螺旋-环-螺旋（bHLH）转录因子在植物生长、发育、代谢、激素信号通路以及对非生物胁迫的响应中发挥着重要作用。然而，目前尚未见关于荒漠真盐生植物中该基因家族的全面基因组和功能分析报道。盐地碱蓬是一种一年生非花环解剖结构的盐生植物，在恶劣的自然生境中具有较高的光合效率，是鉴定参与抗逆转录因子的理想植物。本研究基于保守基序、基因结构和系统发育分析，在盐地碱蓬中鉴定出83个bHLH基因，并将其分为21个亚家族。功能注释富集分析表明，大多数盐地碱蓬bHLH基因在参与应激条件响应的基因本体论（GO）术语和京都基因与基因组百科全书（KEGG）途径中富集，作为转录因子发挥作用。在盐地碱蓬bHLH基因的启动子区域还预测到了一些与植物激素和应激反应相关的顺式作用元件，这在各种非生物胁迫条件（NaCl、甘露醇、低温、ABA、GA、MeJA和SA）下的表达分析中得到了证实；大多数基因参与干旱和盐胁迫耐受性。定位于细胞核的SabHLH169（076）蛋白参与转录活性，其基因表达可能受不同光质的影响。本研究是对盐地碱蓬bHLH基因家族的首次全面分析。这些数据将有助于进一步阐明其在荒漠植物适应非生物胁迫中的分子功能。
