Chen Jingbin, Somta Prakit, Chen Xin, Cui Xiaoyan, Yuan Xingxing, Srinives Peerasak
Institute of Vegetable Crops, Jiangsu Academy of Agricultural SciencesNanjing, China; Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart UniversityNakhon Pathom, Thailand.
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Nakhon Pathom, Thailand.
Front Plant Sci. 2016 Jun 10;7:830. doi: 10.3389/fpls.2016.00830. eCollection 2016.
Mungbean (Vigna radiata L.) is a cleistogamous plant in which flowers are pollinated before they open, which prevents yield improvements through heterosis. We previously generated a chasmogamous mutant (CM) mungbean in which open flowers are pollinated. In this study, we developed insertion/deletion (indel) markers based on the transcriptome differences between CM and Sulu-1 (i.e., normal flowering) plants. An F2 population derived from a cross between CM and Sulu-1 was used for gene mapping. Segregation analyses revealed that a single recessive gene regulates the production of chasmogamous flowers. Using newly developed indel and simple sequence repeat markers, the cha gene responsible for the chasmogamous flower trait was mapped to a 277.1-kb segment on chromosome 6. Twelve candidate genes were detected in this segment, including Vradi06g12650, which encodes a YUCCA family protein associated with floral development. A single base pair deletion producing a frame-shift mutation and a premature stop codon in Vradi06g12650 was detected only in CM plants. This suggested that Vradi06g12650 is a cha candidate gene. Our results provide important information for the molecular breeding of chasmogamous mungbean lines, which may serve as new genetic resources for hybrid cultivar development.
绿豆（Vigna radiata L.）是一种闭花受精植物，其花朵在开放前就已授粉，这阻碍了通过杂种优势提高产量。我们之前培育出了一种开花受精突变体（CM）绿豆，其花朵开放时进行授粉。在本研究中，我们基于CM和苏绿1号（即正常开花）植株之间的转录组差异开发了插入/缺失（indel）标记。利用CM和苏绿1号杂交产生的F2群体进行基因定位。分离分析表明，一个隐性基因调控开花受精花朵的产生。使用新开发的indel和简单序列重复标记，将控制开花受精花性状的cha基因定位到6号染色体上一个277.1 kb的区段。在该区段检测到12个候选基因，包括Vradi06g12650，它编码一种与花发育相关的YUCCA家族蛋白。仅在CM植株中检测到Vradi06g12650中一个导致移码突变和提前终止密码子的单碱基对缺失。这表明Vradi06g12650是cha候选基因。我们的结果为开花受精绿豆品系的分子育种提供了重要信息，这些品系可作为杂交品种开发的新遗传资源。
