Gaulee Pratima, Yang Zhihui, Sura Livia, Xu Haiyan, Rossignol Candace, Weiss Michael D, Bliznyuk Nikolay
Department of Pediatrics, University of Florida, Gainesville, FL, United States.
Department of Emergency Medicine, University of Florida, Gainesville, FL, United States.
Front Neurol. 2022 Jul 7;13:934755. doi: 10.3389/fneur.2022.934755. eCollection 2022.
To determine the concentrations of four neuroprotein biomarkers and 68 miRNAs in neonates with low cord pH and/or mild hypoxic-ischemic encephalopathy (HIE).
A prospective cohort study enrolled neonates with low cord pH ( = 18), moderate-severe HIE ( = 40), and healthy controls ( = 38). Groups provided serum samples at 0-6 h of life. The concentrations of biomarkers and miRNAs were compared between cohorts.
The low cord pH and moderate-severe HIE groups had increased concentrations of GFAP, NFL and Tau compared to controls ( < 0.05, < 0.001, respectively). NFL concentrations in mild HIE was higher than controls ( < 0.05) but less than moderate-severe HIE ( < 0.001). Of 68 miRNAs, 36 in low cord pH group and 40 in moderate-severe HIE were upregulated compared to controls ( < 0.05). Five miRNAs in low cord pH group ( < 0.05) and 3 in moderate-severe HIE were downregulated compared to controls ( < 0.05).
A biomarker panel in neonates with low cord pH may help clinicians make real-time decisions.
测定脐血pH值低和/或轻度缺氧缺血性脑病（HIE）新生儿的四种神经蛋白生物标志物和68种微小RNA（miRNA）的浓度。
一项前瞻性队列研究纳入了脐血pH值低的新生儿（n = 18）、中重度HIE新生儿（n = 40）和健康对照者（n = 38）。各研究组在出生后0 - 6小时提供血清样本。比较各队列之间生物标志物和miRNA的浓度。
与对照组相比，脐血pH值低组和中重度HIE组的胶质纤维酸性蛋白（GFAP）、神经丝轻链（NFL）和微管相关蛋白tau（Tau）浓度升高（分别为P < 0.05，P < 0.001）。轻度HIE组的NFL浓度高于对照组（P < 0.05），但低于中重度HIE组（P < 0.001）。与对照组相比，68种miRNA中，脐血pH值低组有36种、中重度HIE组有40种miRNA上调（P < 0.05）。与对照组相比，脐血pH值低组有5种miRNA下调（P < 0.05），中重度HIE组有3种miRNA下调（P < 0.05）。
脐血pH值低的新生儿生物标志物组可能有助于临床医生做出实时决策。
