Hu Yunxuan, Liu Xiaoze, Wu Xiuna, Zhang Zhengxiao, Wu Daren, Chen Chaoxiang, Su Wenjin, Zhang Lingyu, Li Jian, Wang Hui-Min David
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen 361021, China.
Food Chem X. 2022 Jul 16;15:100390. doi: 10.1016/j.fochx.2022.100390. eCollection 2022 Oct 30.
In this study, we aimed to isolate and identify the bioactive compounds from 5-year pickled radish. The pickled radish was extracted with methanol or ethyl acetate. Sephadex LH-20, normal phase and reverse phase silica gel column chromatography were used for separation and purification, combined with thin layer chromatography (TLC), high performance liquid chromatography (HPLC), electrospray mass spectrometry (ESI-MS), nuclear magnetic resonance spectroscopy (NMR) technology for structural identification. The results showed that 6 compounds were separated and purified from methanol and ethyl acetate extracts of 5-year-old pickled radish. The structures were identified as 5-hydroxymethylfurfural, β-sitosterol, β-sitosterol-3-O-glucose glycosides, α-linolenic acid, 1-monopalmitin and chaenomic acid A. Using molecular docking, it was determined that β-sitosterol and its derivative β-sitosterol-3-O-glucose glycosides have high affinity for five antioxidant enzymes, and there were multiple hydrogen bonds between them. These results indicated that pickled radishes might be used as an important source of natural chemical substances.
在本研究中,我们旨在从5年陈酿的泡菜萝卜中分离并鉴定生物活性化合物。用甲醇或乙酸乙酯对泡菜萝卜进行提取。采用葡聚糖凝胶LH-20、正相和反相硅胶柱色谱进行分离纯化,并结合薄层色谱(TLC)、高效液相色谱(HPLC)、电喷雾质谱(ESI-MS)、核磁共振光谱(NMR)技术进行结构鉴定。结果表明,从5年陈酿泡菜萝卜的甲醇和乙酸乙酯提取物中分离并纯化出6种化合物。其结构被鉴定为5-羟甲基糠醛、β-谷甾醇、β-谷甾醇-3-O-葡萄糖苷、α-亚麻酸、1-单棕榈酸甘油酯和山奈酸A。通过分子对接确定,β-谷甾醇及其衍生物β-谷甾醇-3-O-葡萄糖苷对五种抗氧化酶具有高亲和力,且它们之间存在多个氢键。这些结果表明,泡菜萝卜可能是天然化学物质的重要来源。