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一种使用宽带介电谱测量霍乱毒素 B 五聚体慢蛋白动力学的方案。

A protocol to measure slow protein dynamics of the cholera toxin B pentamers using broadband dielectric spectroscopy.

机构信息

University of Lyon, CNRS, INSA Lyon, Université Claude Bernard Lyon 1, Ecole Centrale de Lyon, Ampère, UMR5005, 69622 Villeurbanne, France; University of Lyon, CNRS, IMP, 69622 Villeurbanne, France.

University of Lyon, CNRS, INSA Lyon, Université Claude Bernard Lyon 1, Ecole Centrale de Lyon, Ampère, UMR5005, 69622 Villeurbanne, France; Institut Rhônalpin des systèmes complexes, IXXI-ENS-Lyon, 69007 Lyon, France.

出版信息

STAR Protoc. 2022 Jul 18;3(3):101561. doi: 10.1016/j.xpro.2022.101561. eCollection 2022 Sep 16.

DOI:10.1016/j.xpro.2022.101561
PMID:35874473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9304676/
Abstract

The present protocol describes how to measure experimentally the slow protein dynamics that take place upon the thermal unfolding of the B subunit cholera toxin pentamers using broadband dielectric spectroscopy (BDS) in weakly hydrated and nanoconfined conditions. Transient unfolding intermediates, rarely identified otherwise, are revealed thanks to the B subunit's remarkable heat resistance up to 180°C and distinct molecular dynamics. The frequencies detected experimentally are consistent with the spatiotemporal scales of motions of molecular dynamics simulation. For complete details on the use and execution of this protocol, please refer to Bourgeat et al. (2021, 2019).

摘要

本方案描述了如何在弱水合和纳米约束条件下使用宽频介电谱（BDS）实验测量 B 亚基霍乱毒素五聚体热解折叠过程中的慢蛋白动力学。由于 B 亚基具有高达 180°C 的惊人耐热性和独特的分子动力学特性，因此可以揭示其他方法很少能识别的瞬态展开中间体。实验检测到的频率与分子动力学模拟的时空运动尺度一致。有关该方案使用和执行的完整详细信息，请参阅 Bourgeat 等人（2021 年，2019 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/3ef840c6e17b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/752e27ff411a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/ec3fef64a34f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/03d900387b64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/abd181b8be7f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/3ef840c6e17b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/752e27ff411a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/ec3fef64a34f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/03d900387b64/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/abd181b8be7f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bac/9304676/3ef840c6e17b/gr4.jpg

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A protocol to measure slow protein dynamics of the cholera toxin B pentamers using broadband dielectric spectroscopy.一种使用宽带介电谱测量霍乱毒素 B 五聚体慢蛋白动力学的方案。
STAR Protoc. 2022 Jul 18;3(3):101561. doi: 10.1016/j.xpro.2022.101561. eCollection 2022 Sep 16.
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本文引用的文献

1
Experimental diagnostic of sequence-variant dynamic perturbations revealed by broadband dielectric spectroscopy.宽带介电谱揭示的序列变异动态扰动的实验诊断
Structure. 2021 Dec 2;29(12):1419-1429.e3. doi: 10.1016/j.str.2021.05.005. Epub 2021 May 28.
2
Stabilization of proteins embedded in sugars and water as studied by dielectric spectroscopy.通过介电谱研究嵌入在糖和水中的蛋白质的稳定性。
Phys Chem Chem Phys. 2020 Sep 30;22(37):21197-21207. doi: 10.1039/d0cp03281f.
3
Experimental Protein Molecular Dynamics: Broadband Dielectric Spectroscopy coupled with nanoconfinement.
实验蛋白质分子动力学：与纳米约束相结合的宽带介电光谱学。
Sci Rep. 2019 Nov 29;9(1):17988. doi: 10.1038/s41598-019-54562-8.
4
In proteins, the structural responses of a position to mutation rely on the Goldilocks principle: not too many links, not too few.在蛋白质中，一个位置的结构响应依赖于适者生存原则：链接既不能太多，也不能太少。
Phys Chem Chem Phys. 2018 Oct 10;20(39):25399-25410. doi: 10.1039/c8cp04530e.
5
In situ monitoring of PTHLH secretion in neuroblastoma cells cultured onto nanoporous membranes.在纳米孔膜上培养的神经母细胞瘤细胞中 PTHLH 分泌的原位监测。
Biosens Bioelectron. 2018 Jun 1;107:62-68. doi: 10.1016/j.bios.2018.01.064. Epub 2018 Feb 3.
6
When fast is better: protein folding fundamentals and mechanisms from ultrafast approaches.何时快速更佳：超快方法揭示的蛋白质折叠基本原理与机制
Biochem J. 2016 Sep 1;473(17):2545-59. doi: 10.1042/BCJ20160107.
7
Nanofluidics Approach to Separate between Static and Kinetic Nanoconfinement Effects on the Crystallization of Polymers.纳米流体制备技术可分离聚合物结晶过程中的静态和动力学纳米限域效应。
Nano Lett. 2015 Jul 8;15(7):4311-6. doi: 10.1021/acs.nanolett.5b00185. Epub 2015 Jun 4.
8
Rhodopsin-lipid interactions studied by NMR.通过核磁共振研究视紫红质与脂质的相互作用。
Methods Enzymol. 2013;522:209-27. doi: 10.1016/B978-0-12-407865-9.00012-1.
9
Curie transitions for attograms of ferroelectric polymers.铁电聚合物的居里转变达到 attograms 级。
Nano Lett. 2013 Feb 13;13(2):577-80. doi: 10.1021/nl304103y. Epub 2013 Jan 22.
10
Nanochannels for diagnostic of thrombin-related diseases in human blood.用于诊断人血液中与凝血酶相关疾病的纳米通道。
Biosens Bioelectron. 2013 Feb 15;40(1):24-31. doi: 10.1016/j.bios.2012.05.021. Epub 2012 May 31.