Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA, USA.
STAR Protoc. 2022 Jul 18;3(3):101558. doi: 10.1016/j.xpro.2022.101558. eCollection 2022 Sep 16.
The nematode is a common model organism for studies in immunity and host-pathogen interactions. In this protocol, we describe techniques for measuring pharyngeal pumping and defecation rates of adult exposed to either pathogenic or non-pathogenic bacteria. Quantifying changes in pumping or defecation rates during pathogenic infection can account for behavioral changes that may affect survival. We also detail how to synchronize and expose the synchronize animals to either pathogenic or non-pathogenic bacteria. For complete details on the use and execution of this protocol, please refer to Wibisono et al. (2022).
线虫是研究免疫和宿主-病原体相互作用的常用模式生物。在本方案中，我们描述了测量暴露于致病性或非致病性细菌的成年线虫的咽泵和排粪率的技术。量化致病性感染过程中泵吸或排粪率的变化，可以解释可能影响生存的行为变化。我们还详细介绍了如何同步线虫并将同步后的线虫暴露于致病性或非致病性细菌中。有关本方案使用和执行的完整详细信息，请参阅 Wibisono 等人。（2022 年）。
