Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, 70013 Crete, Greece; Department of Basic Sciences, Medical School, University of Crete, Heraklion, 71003 Crete, Greece.
Department of Toxicology, Medical School, University of Crete, Heraklion, 71003 Crete, Greece.
STAR Protoc. 2024 Mar 15;5(1):102801. doi: 10.1016/j.xpro.2023.102801. Epub 2023 Dec 29.
C. elegans is a bacteria-eating soil-dwelling nematode. Typical cultivation of laboratory-reared populations occurs on bacteria-covered solid media, where they move along with sinusoidal undulations. Nematodes decelerate when they encounter food. Dopaminergic and serotonergic neurotransmission regulate this behavior. Here, we describe the procedure for determining food-dependent locomotion rate of fed and fasting nematodes. We detail steps for assay plate preparation, C. elegans synchronization, and assessment of locomotion. The behaviors we describe provide information regarding the animal's physiological neuronal and muscular function. For complete details on the use and execution of this protocol, please refer to Petratou et al. (2023) and Sawin et al. (2000)..
秀丽隐杆线虫是一种以细菌为食的土栖线虫。实验室培养的秀丽隐杆线虫通常在覆盖有细菌的固体培养基上进行培养，它们在培养基上沿着正弦曲线波动移动。当线虫遇到食物时，它们会减速。多巴胺能和血清素能神经递质调节这种行为。在这里，我们描述了确定喂食和禁食线虫对食物依赖性运动速度的方法。我们详细介绍了测定板的制备、秀丽隐杆线虫的同步化以及运动评估的步骤。我们描述的行为提供了有关动物生理神经元和肌肉功能的信息。有关该方案的使用和执行的完整详细信息，请参阅 Petratou 等人（2023 年）和 Sawin 等人（2000 年）。
