The School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China.
J Immunol Res. 2022 Jul 15;2022:7277583. doi: 10.1155/2022/7277583. eCollection 2022.
N6-methyladenosine (mA) is the most common epigenetic modification existing in eukaryocyte transcripts. However, genes related to mA modification in oral squamous cell carcinoma (OSCC) are still unclear. Here, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to map the m6A landscape in OSCC and corresponding controls. The mA peaks are always distributed in the junction of the 3'-untranslated regions (3'-UTRs) and the coding sequences (CDS) of mRNAs, as well as the entire genome of long noncoding RNA (lncRNA). Furthermore, enrichment analysis showed that differentially methylated genes were significantly enriched in NF-kappa B signaling pathway, Hedgehog signaling pathway, etc. In summary, our findings reveal the landscape of mA modification on mRNAs and lncRNAs in OSCC, which may provide key clues for the precision-targeted therapy of OSCC.
N6-甲基腺苷（mA）是真核细胞转录本中最常见的表观遗传修饰。然而，口腔鳞状细胞癌（OSCC）中与 mA 修饰相关的基因仍不清楚。在这里，进行了甲基化 RNA 免疫沉淀测序（MeRIP-Seq）以绘制 OSCC 及相应对照中 m6A 的图谱。mA 峰总是分布在信使 RNA（mRNA）的 3'-非翻译区（3'-UTRs）和编码序列（CDS）的交界处，以及长非编码 RNA（lncRNA）的整个基因组中。此外，富集分析表明，差异甲基化基因在 NF-κB 信号通路、Hedgehog 信号通路等中显著富集。总之，我们的研究结果揭示了 OSCC 中 mA 修饰在 mRNA 和 lncRNA 上的图谱，这可能为 OSCC 的精准靶向治疗提供关键线索。
