Chlamydia trachomatis from men with non-gonococcal urethritis. Simplified procedure for cultivation and isolation in replicating McCoy cell culture.

Chlamydia trachromatis was cultivated on replicating McCoy cells without the addition of antimetabolites. A further technical modification was centrifugation of the specimens at room temperature at 4000 g, thus making it possible to use the method in any microbiological laboratory. C. trachomatis was isolated from 36 of 81 patients (44.4%) with non-gonococcal urethritis. This rate compares well with reported isolation rates using antireplicative agents and higher centrifugation temperatures.