Šimunović Katarina, Sahin Orhan, Erega Andi, Štefanič Polonca, Zhang Qijing, Mandic Mulec Ines, Smole Možina Sonja, Klančnik Anja
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Department of Microbiology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Front Microbiol. 2022 Jul 8;13:910616. doi: 10.3389/fmicb.2022.910616. eCollection 2022.
is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source of infection. To reduce the risk associated with transmission via poultry meat, effective interventions during poultry production are needed, and the use of probiotics is a promising approach. In this study, 15 strains were initially screened for their anti- activities. PS-216 strain demonstrated the best anti- activity against 15 isolates when examined using co-cultures. To evaluate the suitability of PS-216 for probiotic use, its susceptibility to eight clinically important antimicrobials and simulated gastric conditions was investigated. PS-216 was sensitive to all of the tested antibiotics. Although vegetative cells were sensitive to gastric conditions, PS-216 spores were highly resistant. We further evaluated the use of a PS-216 spore preparation (2.5 × 10 CFU/mL water) to prevent and/or reduce colonization in broiler chickens . Compared to the untreated group, significantly lower counts were detected in caeca of broilers continuously treated with PS-216 spores in their drinking water. Furthermore, broilers continuously treated with PS-216 spores showed improved weight gain, compared to the control group. Together, these results demonstrate the potential of PS-216 for use in poultry to reduce colonization and improve weight gain.
是全球人类细菌性肠胃炎（即弯曲杆菌病）的主要病因，家禽是主要感染源。为降低通过禽肉传播相关风险，家禽生产过程中需要有效的干预措施，而使用益生菌是一种很有前景的方法。在本研究中，最初筛选了15株菌株的抗菌活性。使用共培养法检测时，PS - 216菌株对15株分离株表现出最佳抗菌活性。为评估PS - 216作为益生菌使用的适用性，研究了其对八种临床重要抗菌药物的敏感性以及模拟胃部环境的耐受性。PS - 216对所有测试抗生素敏感。虽然营养细胞对胃部环境敏感，但PS - 216芽孢具有高度抗性。我们进一步评估了PS - 216芽孢制剂（2.5×10 CFU/mL水）预防和/或减少肉鸡中（弯曲杆菌）定植的效果。与未处理组相比，在饮用水中持续添加PS - 216芽孢的肉鸡盲肠中检测到的（弯曲杆菌）数量显著更低。此外，与对照组相比，持续用PS - 216芽孢处理的肉鸡体重增加情况更好。总之，这些结果证明了PS - 216在家禽中用于减少（弯曲杆菌）定植和提高体重增加的潜力。 （注：原文中“2.5 × 10 CFU/mL”表述似乎不完整，推测可能是“2.5 × 10⁸CFU/mL”之类，这里按原文翻译。）
