Pang Jinji, Looft Torey, Zhang Qijing, Sahin Orhan
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
National Animal Disease Center, United States Department of Agriculture, Ames, IA 50010, USA.
Microorganisms. 2023 Jun 30;11(7):1724. doi: 10.3390/microorganisms11071724.
is a major food safety concern and is transmitted mainly via poultry meat. We previously found that some commercial broiler farms consistently produced -negative flocks while others were consistently -positive for consecutive production cycles although the farms operated under similar management practices. We hypothesized that this difference in colonization might be associated with the gut microbiota composition. To address this, six commercial broiler farms were selected based on their status (three negative and three positive) to evaluate the microbiota differences between each farm category. For each farm on each production cycle (2-3 cycles), 40 ceca collected from five-week-old broilers were processed for microbiota analysis via 16S rRNA gene sequencing. Cecal microbiota species richness, phylogenetic diversity, community structure, and composition of -positive farms were noticeably different from those of -negative farms. , , , , and were significantly more abundant among -positive farms. In contrast, , , , , , , and were more abundant in -negative farms. , , , , , and were significantly negatively correlated with abundance. These findings suggest that specific members of cecal microbiota may influence colonization in commercial broilers and may be further explored to control in poultry.
是一个主要的食品安全问题，主要通过禽肉传播。我们之前发现，一些商业肉鸡养殖场持续产出阴性鸡群，而其他养殖场在连续的生产周期中则持续呈阳性，尽管这些养殖场在相似的管理方式下运营。我们推测这种在[某种细菌]定殖方面的差异可能与肠道微生物群组成有关。为了解决这个问题，根据它们的[某种细菌]状态（三个阴性和三个阳性）选择了六个商业肉鸡养殖场，以评估每个养殖场类别之间的微生物群差异。在每个生产周期（2 - 3个周期），从每个养殖场选取五周龄肉鸡的40个盲肠，通过16S rRNA基因测序对其进行微生物群分析。阳性养殖场的盲肠微生物群物种丰富度、系统发育多样性、群落结构和组成与阴性养殖场明显不同。[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]、[具体细菌名称4]和[具体细菌名称5]在阳性养殖场中显著更为丰富。相比之下，[具体细菌名称6]、[具体细菌名称7]、[具体细菌名称8]、[具体细菌名称9]、[具体细菌名称10]、[具体细菌名称11]和[具体细菌名称12]在阴性养殖场中更为丰富。[具体细菌名称6]、[具体细菌名称7]、[具体细菌名称8]、[具体细菌名称9]、[具体细菌名称10]和[具体细菌名称11]与[某种细菌]丰度显著负相关。这些发现表明，盲肠微生物群的特定成员可能影响商业肉鸡中[某种细菌]的定殖，并且可能需要进一步探索以控制家禽中的[某种细菌]。
