Chun Seong-Jun, Cui Yingshun, Yoo Su-Hyang, Lee Jung Ro
LMO Team, National Institute of Ecology, Seocheon, South Korea.
Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
Front Microbiol. 2022 Jul 8;13:920759. doi: 10.3389/fmicb.2022.920759. eCollection 2022.
(Rapeseed) is an econfomically important oil-producing crop. The microbial interactions in the plant holobiont are fundamental to the understanding of plant growth and health. To investigate the microbial dynamics in the holobiont of feral , a total of 215 holobiont samples, comprised of bulk soil, primary root, lateral root, dead leaf, caulosphere, basal leaf, apical leaf, carposphere, and anthosphere, were collected from five different grassland sites in South Korea. The soil properties differed in different sampling sites, but prokaryotic communities were segregated according to plant holobiont components. The structures of the site-specific SparCC networks were similar across the regions. Recurrent patterns were found in the plant holobionts in the recurrent network. sp., sp., and clusters were observed consistently and were identified as core taxa in the phyllosphere, dead leaf microbiome, and rhizosphere, respectively. Arthropod-related microbes, such as sp., sp., and Corynebacteriales amplicon sequence variants, were found in the anthosphere. PICRUSt2 analysis revealed that microbes also possessed specific functions related to holobiont components, such as functions related to degradation pathways in the dead leaf microbiome. Structural equation modeling analysis showed the organic connections among holobiont components and the essential roles of the core microbes in the holobiont formations in natural ecosystem. Microbes coexisting in a specific plant showed relatively stable community structures, even though the regions and soil characteristics were different. Microbes in each plant component were organically connected to form their own plant holobiont. In addition, plant-related microbes, especially core microbes in each holobiont, showed recurrent interaction patterns that are essential to an understanding of the survival and coexistence of plant microbes in natural ecosystems.
油菜是一种具有重要经济价值的产油作物。植物全生物中的微生物相互作用对于理解植物生长和健康至关重要。为了研究野生油菜全生物中的微生物动态，从韩国五个不同的草地站点采集了总共215个全生物样本，包括土壤、主根、侧根、枯叶、茎际、基生叶、顶生叶、果际和花际。不同采样地点的土壤性质不同，但原核生物群落根据植物全生物成分而分离。特定地点的SparCC网络结构在不同区域相似。在循环网络中的植物全生物中发现了循环模式。分别在叶际、枯叶微生物群和根际中一致观察到了sp.、sp.和簇，并将其鉴定为核心分类群。在花际中发现了与节肢动物相关的微生物，如sp.、sp.和棒杆菌属扩增子序列变体。PICRUSt2分析表明，微生物还具有与全生物成分相关的特定功能，如枯叶微生物群中与降解途径相关的功能。结构方程模型分析显示了全生物成分之间的有机联系以及核心微生物在自然生态系统全生物形成中的重要作用。即使区域和土壤特征不同，共存于特定植物中的微生物群落结构相对稳定。每个植物成分中的微生物有机连接形成各自的植物全生物。此外，与植物相关的微生物，尤其是每个全生物中的核心微生物，表现出循环相互作用模式，这对于理解植物微生物在自然生态系统中的生存和共存至关重要。
