Department of Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany.
Institute of Virology and Immunology (IVI), Bern, Switzerland.
Autophagy. 2023 Feb;19(2):731-733. doi: 10.1080/15548627.2022.2100617. Epub 2022 Jul 24.
The recurrence of zoonotic transmission events highlights the need for novel treatment strategies against emerging coronaviruses (CoVs), namely SARS-CoV, MERS-CoV and most notably SARS-CoV-2. Our recently performed genome-wide CRISPR knockout screen revealed a list of conserved pan-coronavirus as well as MERS-CoV or HCoV-229E-specific host dependency factors (HDF) essential during the viral life cycle. Intriguingly, we identified the macroautophagy/autophagy pathway-regulating immunophilins FKBP8, TMEM41B, and MINAR1 as conserved MERS-CoV, HCoV-229E, SARS-CoV, and SARS-CoV-2 host factors, which further constitute potential targets for therapeutic intervention by clinically approved drugs.
人畜共患病传播事件的反复发生，凸显了针对新兴冠状病毒（CoV），即 SARS-CoV、MERS-CoV 以及更值得关注的 SARS-CoV-2，研发新型治疗策略的必要性。我们最近进行的全基因组 CRISPR 敲除筛选揭示了一组保守的泛冠状病毒以及 MERS-CoV 或 HCoV-229E 特异性宿主依赖性因子（HDF），这些因子在病毒生命周期中是必不可少的。有趣的是，我们鉴定出了调控巨自噬/自噬途径的免疫亲和素 FKBP8、TMEM41B 和 MINAR1，它们是保守的 MERS-CoV、HCoV-229E、SARS-CoV 和 SARS-CoV-2 的宿主因子，这些因子进一步构成了临床批准药物进行治疗干预的潜在靶点。
