Shan Youxia, Zhang Dandan, Luo Zisheng, Li Taotao, Qu Hongxia, Duan Xuewu, Jiang Yueming
Guangdong Provincial Key Laboratory of Applied Botany, Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Compr Rev Food Sci Food Saf. 2022 Sep;21(5):4251-4273. doi: 10.1111/1541-4337.13003. Epub 2022 Jul 25.
Due to the global use of cold chain, the development of postharvest technology to reduce chilling injury (CI) in postharvest fruits and vegetables during storage and transport is needed urgently. Considerable evidence shows that maintaining intracellular adenosine triphosphate (iATP) in harvested fruits and vegetables is beneficial to inhibiting CI occurrence. Extracellular ATP (eATP) is a damage-associated signal molecule and plays an important role in CI of postharvest fruits and vegetables through its receptor and subsequent signal transduction under low-temperature stress. The development of new aptasensors for the simultaneous determination of eATP level allows for better understanding of the roles of eATP in a myriad of responses mediated by low-temperature stress in relation to the chilling tolerance of postharvest fruits and vegetables. The multiple biological functions of eATP and its receptors in postharvest fruits and vegetables were attributed to interactions with reactive oxygen species (ROS) and nitric oxide (NO) in coordination with phytohormones and other signaling molecules via downstream physiological activities. The complicated interconnection among eATP in relation to its receptors, eATP/iATP homeostasis, ROS, NO, and heat shock proteins triggered by eATP recognition has been emphasized. This paper reviews recent advances in the beneficial effects of energy handling, outlines the production and homeostasis of eATP, discusses the possible mechanism of eATP and its receptors in chilling tolerance, and provides future research directions for CI in postharvest fruits and vegetables during low-temperature storage.
由于冷链的全球使用，迫切需要开发采后技术以减少采后果蔬在储存和运输过程中的冷害（CI）。大量证据表明，维持采后果蔬中的细胞内三磷酸腺苷（iATP）有利于抑制冷害的发生。细胞外ATP（eATP）是一种损伤相关信号分子，在低温胁迫下通过其受体及后续信号转导，在采后果蔬的冷害中发挥重要作用。开发用于同时测定eATP水平的新型适配体传感器，有助于更好地理解eATP在采后果蔬低温胁迫介导的多种反应中与耐冷性相关的作用。eATP及其受体在采后果蔬中的多种生物学功能归因于其与活性氧（ROS）和一氧化氮（NO）的相互作用，并通过下游生理活动与植物激素和其他信号分子协同作用。eATP与其受体、eATP/iATP稳态、ROS、NO以及由eATP识别引发的热休克蛋白之间复杂的相互联系已得到强调。本文综述了能量处理的有益作用的最新进展，概述了eATP的产生和稳态，讨论了eATP及其受体在耐冷性方面的可能机制，并为低温储存期间采后果蔬的冷害提供了未来的研究方向。
