Huang Shuai, Bi Ying, Li Hui, Liu Caihong, Wang Xue, Wang Xinyu, Lei Yaxin, Zhang Qi, Wang Jing
College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China.
Foods. 2023 May 6;12(9):1904. doi: 10.3390/foods12091904.
To investigate the effect of -butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo 'Hami'), the fruits were soaked in a 1.0% solution of -butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly, and the indices related to permeability and membrane lipid metabolism of pericarp cells were measured. The results showed that treatment with -butanol inhibited the increase in chilling injury index, membrane permeability, and malondialdehyde content of Hami melon fruits, promoted an increase in the contents of phosphatidyl alcohol and unsaturated fatty acids, such as linoleic acid, linolenic acid, oleic acid (except 14 d), and erucic acid (28-42 d), and decreased the content of saturated fatty acids, stearic acid (0-28 d), phosphatidic acid (except for 21 d), and the key enzymes of membrane lipid metabolism compared with the control. The activities of phospholipase D (PLD) and lipoxygenase (LOX) and the downregulation of the levels of expression and (42 d only) genes reduced the chilling injury index of Hami melon and alleviated the further expansion of chilling injury symptoms in the fruits. We also cloned the key gene of membrane lipid metabolism , which was obtained by pre-transcriptome screening of the pericarp. We found that of Hami melon had the closest affinity with cucumber (CsXP5), indicating that the gene of Hami melon was functionally similar to that of cucumber. In addition, a two-fold alignment analysis of and CmXP5 base sequences indicated that the base sequences of the two promoter regions differed from each other.
为研究正丁醇对哈密瓜（甜瓜‘哈密’）采后膜脂代谢的影响，以水为对照，将果实浸泡在1.0%的正丁醇溶液中30分钟。定期观察冷害症状，并测定与果皮细胞通透性和膜脂代谢相关的指标。结果表明，正丁醇处理抑制了哈密瓜果实冷害指数、膜通透性和丙二醛含量的增加，促进了磷脂酰乙醇和不饱和脂肪酸（如亚油酸、亚麻酸、油酸（14天除外）和芥酸（28 - 42天））含量的增加，降低了饱和脂肪酸、硬脂酸（0 - 28天）、磷脂酸（21天除外）以及与对照相比膜脂代谢关键酶的含量。磷脂酶D（PLD）和脂氧合酶（LOX）的活性以及基因和（仅42天）表达水平的下调降低了哈密瓜的冷害指数，减轻了果实冷害症状的进一步扩展。我们还克隆了膜脂代谢关键基因，该基因是通过对果皮进行转录组预筛选获得的。我们发现哈密瓜的与黄瓜（CsXP5）具有最密切的亲和力，表明哈密瓜的基因在功能上与黄瓜的相似。此外，和CmXP5碱基序列的双重比对分析表明，两个启动子区域的碱基序列彼此不同。
