Han Qianqian, Wang Xuan, Qiu Lin, Zhou Xinpei, Hui Zexuan, Ni Xinye, Xuan Yang, Lei Xiaoling, Wang Jianhao
School of Pharmacy, Changzhou University, Changzhou 213164, China.
The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou 213003, China.
Gels. 2022 Jun 23;8(7):397. doi: 10.3390/gels8070397.
The unique bactericidal mechanism of metal nanoparticles (MNPs) is considered to be an effective strategy to deal with antibiotic resistance, but the oxidative stress damage caused by excessive accumulation of MNPs to normal cells cannot be ignored. Achieving on-demand release of nano-drugs in specific infection environments is highly attractive. Herein, we constructed a "core-shell" nanogel (G@CuS) based on a copper sulfide (CuS) antimicrobial agent and gelatin for targeted drug release and bacterial clearance in a gelatinase infected microenvironment. G@CuS produced heat and reactive oxygen species (ROS) under the irradiation of a laser, which together with the released Cu cause irreversible and efficient physical damage to the bacteria. Moreover, the encapsulation of gelatin not only limits the biotoxicity of CuS nanodots (NDs), but also effectively promotes the proliferation of mammalian cells. Under the synergy of multiple mechanisms, G@CuS eradicated the colonized bacteria in the wound of mice infected with () and accelerated wound healing. The proposed application strategy of nanogel is expected to provide a new idea for clinical transformation.
金属纳米颗粒（MNPs）独特的杀菌机制被认为是应对抗生素耐药性的有效策略，但MNPs过度积累对正常细胞造成的氧化应激损伤不容忽视。在特定感染环境中实现纳米药物的按需释放极具吸引力。在此，我们基于硫化铜（CuS）抗菌剂和明胶构建了一种“核壳”纳米凝胶（G@CuS），用于在明胶酶感染的微环境中实现靶向药物释放和细菌清除。G@CuS在激光照射下产生热量和活性氧（ROS），它们与释放出的铜一起对细菌造成不可逆且高效的物理损伤。此外，明胶的包裹不仅限制了CuS纳米点（NDs）的生物毒性，还有效促进了哺乳动物细胞的增殖。在多种机制的协同作用下，G@CuS根除了感染（）的小鼠伤口中定植的细菌，并加速了伤口愈合。所提出的纳米凝胶应用策略有望为临床转化提供新思路。
