Centre for Biotechnology and Bioengineering (CeBiB), Center of Food Biotechnology and Bioseparations, BIOREN and Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar 01145, Temuco 4780000, Chile.
School of Engineering, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
Mar Drugs. 2022 Jun 25;20(7):416. doi: 10.3390/md20070416.
The carotenogenic thraustochytrid sp. RT2316-16 was grown in batch and repeated-batch cultures using different feeds containing glucose, or glycerol, and yeast extract, for the production of lipids, phospholipids and carotenoids. RT2316-16 produced canthaxanthin, astaxanthin and β-carotene. The effects of biotin, ascorbic acid, light and temperature were evaluated in some of the experiments. In 2-day-old batch cultures, the combined mass percentage of eicosapentaenoic acid and docosahexaenoic acid in total lipids was between 16.5% (glycerol-based medium in the dark; biomass concentration = 4.2 ± 1.1 g L) and 42.6% (glucose-based medium under light; biomass concentration = 3.3 ± 0.1 g L), decreasing to 3.8% and 6.1%, respectively, after day 4. In repeated-batch cultures, the total lipids in the biomass increased after glucose or glycerol was fed alone, whereas the total carotenoids (168 ± 7 μg g dry weight (DW)) and phospholipids in the biomass increased after feeding with yeast extract. The biomass with the highest content of phospholipids (28.7 ± 4.3 mg g DW) was obtained using a feed medium formulated with glycerol, yeast extract and ascorbic acid. Glycerol was the best carbon source for the production of a biomass enriched with total lipids (467 ± 45 mg g DW). The composition of carotenoids depended strongly on the composition of the feed. Repeated-batch cultures fed with yeast extract contained canthaxanthin as the main carotenoid, whereas in the cultures fed only with glucose, the biomass contained mainly β-carotene.
采用含有葡萄糖或甘油以及酵母提取物的不同饲料，在分批和重复分批培养中培养产类胡萝卜素的硫球红游动菌 RT2316-16，以生产脂质、磷脂和类胡萝卜素。RT2316-16 产生角黄素、虾青素和β-胡萝卜素。在一些实验中评估了生物素、抗坏血酸、光照和温度的影响。在 2 天龄的分批培养中，总脂质中二十碳五烯酸和二十二碳六烯酸的组合质量百分比在 16.5%（黑暗中基于甘油的培养基；生物质浓度=4.2±1.1 g/L）和 42.6%（光照下基于葡萄糖的培养基；生物质浓度=3.3±0.1 g/L）之间，第 4 天后分别降至 3.8%和 6.1%。在重复分批培养中，单独添加葡萄糖或甘油后生物质中的总脂质增加，而添加酵母提取物后生物质中的总类胡萝卜素（168±7μg/g 干重（DW））和磷脂增加。在用甘油、酵母提取物和抗坏血酸配制的饲料培养基中获得的生物量具有最高含量的磷脂（28.7±4.3mg/g DW）。甘油是生产富含总脂质的生物量（467±45mg/g DW）的最佳碳源。类胡萝卜素的组成强烈依赖于饲料的组成。用酵母提取物喂养的重复分批培养物含有角黄素作为主要类胡萝卜素，而仅用葡萄糖喂养的培养物中，生物质主要含有β-胡萝卜素。
