Thanapimmetha Anusith, Suwaleerat Tharatron, Saisriyoot Maythee, Chisti Yusuf, Srinophakun Penjit
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Bioprocess Biosyst Eng. 2017 Jan;40(1):133-143. doi: 10.1007/s00449-016-1681-y. Epub 2016 Sep 19.
Production of carotenoids by Rhodococcus opacus PD630 is reported. A modified mineral salt medium formulated with glycerol as an inexpensive carbon source was used for the fermentation. Ammonium acetate was the nitrogen source. A dry cell mass concentration of nearly 5.4 g/L could be produced in shake flasks with a carotenoid concentration of 0.54 mg/L. In batch culture in a 5 L bioreactor, without pH control, the maximum dry biomass concentration was ~30 % lower than in shake flasks and the carotenoids concentration was 0.09 mg/L. Both the biomass concentration and the carotenoids concentration could be raised using a fed-batch operation with a feed mixture of ammonium acetate and acetic acid. With this strategy, the final biomass concentration was 8.2 g/L and the carotenoids concentration was 0.20 mg/L in a 10-day fermentation. A control of pH proved to be unnecessary for maximizing the production of carotenoids in this fermentation.
据报道，红平红球菌PD630可生产类胡萝卜素。采用以甘油作为廉价碳源配制的改良无机盐培养基进行发酵。以醋酸铵作为氮源。在摇瓶中可产生近5.4 g/L的干细胞质量浓度，类胡萝卜素浓度为0.54 mg/L。在5 L生物反应器中进行分批培养，不控制pH值，最大干生物量浓度比摇瓶中低约30%，类胡萝卜素浓度为0.09 mg/L。使用醋酸铵和醋酸的进料混合物进行补料分批操作，可提高生物量浓度和类胡萝卜素浓度。采用该策略，在10天的发酵过程中，最终生物量浓度为8.2 g/L，类胡萝卜素浓度为0.20 mg/L。事实证明，在该发酵过程中，控制pH值对于最大限度地生产类胡萝卜素并非必要。
