Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Toxins (Basel). 2022 Jun 29;14(7):440. doi: 10.3390/toxins14070440.
The fungal secondary metabolite patulin is a mycotoxin widespread in foods and beverages which poses a serious threat to human health. However, no enzyme was known to be able to degrade this mycotoxin. For the first time, we discovered that a manganese peroxidase (MnP) from can efficiently degrade patulin. The MnP gene was cloned into pPICZα(A) and then the recombinant plasmid was transformed into X-33. The recombinant strain produced extracellular manganese peroxidase with an activity of up to 3659.5 U/L. The manganese peroxidase MnP was able to rapidly degrade patulin, with hydroascladiol appearing as a main degradation product. Five mg/L of pure patulin were completely degraded within 5 h. Moreover, up to 95% of the toxin was eliminated in a simulated patulin-contaminated apple juice after 24 h. Using as a model, it was demonstrated that the deconstruction of patulin led to detoxification. Collectively, these traits make MnP an intriguing candidate useful in enzymatic detoxification of patulin in foods and beverages.
真菌次生代谢产物棒曲霉素是一种广泛存在于食品和饮料中的真菌毒素，对人类健康构成严重威胁。然而，目前还没有已知的酶能够降解这种真菌毒素。我们首次发现，来自 的锰过氧化物酶（MnP）能够有效地降解棒曲霉素。将 MnP 基因克隆到 pPICZα(A)中，然后将重组质粒转化到 X-33 中。重组菌株产生的胞外锰过氧化物酶活性高达 3659.5 U/L。锰过氧化物酶 MnP 能够快速降解棒曲霉素，主要降解产物为氢过氧麦角甾醇。5mg/L 的纯棒曲霉素在 5 小时内完全降解。此外，在 24 小时后，模拟污染的苹果汁中高达 95%的毒素被消除。以 为模型，证明了棒曲霉素的解构导致了脱毒。总的来说，这些特性使得 MnP 成为一种有吸引力的候选酶，可用于食品和饮料中棒曲霉素的酶法解毒。
