枯草芽孢杆菌孢子表面展示增强了锰过氧化物酶的稳定性和抗逆性。
Bacillus subtilis spore surface display enhances manganese peroxidase stability and stress resistance.
作者信息
He Lu, Ullah Mati, Naeem Muhammad, Ni Zhong, Feng Yong, Shah Tawaf Ali, Assefa Molalign, Almaary Khalid S, Chen Huayou
机构信息
School of the Life Sciences, Jiangsu University, Zhenjiang, Jiangsu Province, China.
College of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
出版信息
Bioresour Bioprocess. 2025 Jun 10;12(1):57. doi: 10.1186/s40643-025-00901-9.
UNLABELLED
Manganese peroxidase (MnP) is in increasing demand due to its ability to degrade straw lignin and facilitate the conversion of agricultural waste into valuable feed resources. However, free MnP faces limitations in industrial applications owing to high operational costs, poor stability, and poor resistance to environmental stressors. In this study, MnP derived from was displayed on the surface of spores using the cotB protein as an anchor molecule. Compared with refolded MnP expressed in , the spore-displayed MnP exhibited superior acid resistance, with an optimal pH of 3.5 (compared to pH 4.0 for the free enzyme). Additionally, the immobilized enzyme CotB-MnP retained 73.9% of its initial activity after incubation at 30℃ for 1 h incubation. Notably, immobilization eliminated the inhibitory effects of K⁺ and Zn²⁺ ions, which instead promoted the activity of the spore-displayed enzyme. The reusable spores maintained 69% of their initial activity after three consecutive cycles, highlighting their potential for industrial straw feed processing.
GRAPHICAL ABSTRACT
[Image: see text]
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1186/s40643-025-00901-9.
未标记
锰过氧化物酶（MnP）因其降解秸秆木质素以及促进农业废弃物转化为有价值饲料资源的能力而需求日益增加。然而，游离的MnP由于运营成本高、稳定性差以及对环境应激源的耐受性差，在工业应用中面临局限性。在本研究中，利用cotB蛋白作为锚定分子，将来源于[具体来源未给出]的MnP展示在[具体菌种未给出]孢子表面。与在[具体表达体系未给出]中表达并复性的MnP相比，孢子展示的MnP表现出更强的耐酸性，其最适pH为3.5（游离酶的最适pH为4.0）。此外，固定化酶CotB-MnP在30℃孵育1小时后保留了其初始活性的73.9%。值得注意的是，固定化消除了K⁺和Zn²⁺离子的抑制作用，相反，这些离子促进了孢子展示酶的活性。可重复使用的孢子在连续三个循环后仍保持其初始活性的69%，突出了它们在工业秸秆饲料加工中的潜力。
图形摘要
[图片：见正文]
补充信息
在线版本包含可在10.1186/s40643-025-00901-9获取的补充材料。
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