Ivan Talida, Matei Ioana Adriana, Novac Cristiana Ștefania, Kalmár Zsuzsa, Borșan Silvia-Diana, Panait Luciana-Cătălina, Gherman Călin Mircea, Ionică Angela Monica, Papuc Ionel, Mihalca Andrei Daniel
Department of Semiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Department of Microbiology, Immunology and Epidemiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Vet Sci. 2022 Jul 8;9(7):343. doi: 10.3390/vetsci9070343.
Tickborne bacterial pathogens have been described worldwide as risk factors for both animal and human health. Spotted fevers caused by Rickettsiae may cause non-specific symptoms, which make clinical diagnosis difficult. The aim of the current study was to evaluate and review the diversity of SFG Rickettsiae in ticks collected in 41 counties in Romania. A total of 2028 questing and engorged ticks collected in Romania belonging to five species were tested by PCR amplification of spp. gltA and 17-D gene fragments: ( = 1128), ( = 507), ( = 165), ( = 128) and ( = 100). Five species were identified following DNA sequence analysis: , , , , and The most common species detected was . Moreover, was detected for the first time in Romania and in ticks. The detection of and in questing larvae of suggests the possible transovarial transmission of these species in ticks. The detection of for the first time in Romania increases the reported SFG diversity in the country.
蜱传细菌病原体在全球范围内被认为是影响动物和人类健康的风险因素。立克次氏体引起的斑点热可能会导致非特异性症状，这使得临床诊断变得困难。本研究的目的是评估和回顾罗马尼亚41个县采集的蜱中斑点热群立克次氏体的多样性。通过对gltA基因和17 - D基因片段进行PCR扩增，对罗马尼亚采集的属于五个物种的总共2028只搜寻蜱和饱血蜱进行了检测：蓖子硬蜱（n = 1128）、全沟硬蜱（n = 507）、嗜群血蜱（n = 165）、长角血蜱（n = 128）和微小牛蜱（n = 100）。通过DNA序列分析鉴定出了五个立克次氏体物种：立氏立克次氏体、西伯利亚立克次氏体、康氏立克次氏体、小蛛立克次氏体和斯洛伐克立克次氏体。检测到的最常见物种是立氏立克次氏体。此外，斯洛伐克立克次氏体在罗马尼亚和蓖子硬蜱中首次被检测到。在全沟硬蜱的搜寻幼虫中检测到康氏立克次氏体和小蛛立克次氏体表明这些立克次氏体物种在蜱中可能存在经卵传播。斯洛伐克立克次氏体在罗马尼亚的首次检测增加了该国已报道的斑点热群立克次氏体的多样性。
