Potgieter F T, van Rensburg L
Onderstepoort J Vet Res. 1987 Mar;54(1):5-7.
Anaplasma centrale was isolated from a field collection of Rhipicephalus simus. Transstadial transmission of A. centrale with adult ticks was demonstrated, but the infection was not carried transovarially. Ticks from this collection were subsequently reared as a non-infected, laboratory strain. It was proved that the Onderstepoort live blood vaccine strain of A. centrale, isolated by Theiler in 1911, is still tick transmissible after more than 75 years of needle passage through cattle in the laboratory. Attempts to demonstrate transstadial transmission of the vaccine strain with Boophilus decoloratus and Boophilus microplus failed.
从采自野外的西门革蜱中分离出中央无形体。已证实中央无形体可经成年蜱进行期间传播，但不会经卵传播。随后，将此次采集的蜱饲养为未感染的实验室菌株。事实证明，1911年由泰勒分离出的中央无形体 Onderstepoort 活血液疫苗菌株，在实验室经牛体连续接种针传75年以上后，仍可通过蜱传播。用消色牛蜱和微小牛蜱证明疫苗菌株期间传播的尝试未成功。
