Laboratorio de Medicina Genómica, Hospital Regional "1° de Ocutbre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado (ISSSTE), Mexico City, Mexico.
Scientific Management of KPC Biotech. California, USA.
J Int Med Res. 2022 Jul;50(7):3000605221099458. doi: 10.1177/03000605221099458.
To investigate the antibody response to SARS-CoV-2 and identify associated factors in frontline and second-line healthcare workers (HCWs) at a large hospital in Mexico City during the first wave of COVID-19 pandemic.
This was a cross-sectional study of HCWs returning to work following mandatory isolation after recovering from COVID-19. Immunoglobulin (Ig) M and IgG antibodies elicited by SARS-CoV-2 were semiquantitatively measured using densitometric analysis of band intensities in lateral flow assay (LFA) devices. The mean pixel intensity (dots-per-inch [dpi]) of each band on the LFA was considered a measure of antibody titre.
Of the 111 HCWs involved in the study, antibody responses were detected in 73/111 (66%) participants. Severe COVID symptoms was associated with old age. No differences in IgM intensity were observed between men and women, but IgG intensity was significantly higher in men than in women. Second-line HCWs produced a higher IgG intensity than firstline HCWs. The IgG intensity was high in severe cases.
For HCWs who may acquire SARS-CoV-2 infection, it is necessary to establish a routine program for detection of the virus to avoid risk of infection and spread of COVID-19.
在墨西哥城一家大型医院的 COVID-19 大流行第一波期间，调查一线和二线医护人员（HCW）对 SARS-CoV-2 的抗体反应，并确定相关因素。
这是一项横断面研究，对从 COVID-19 中康复后强制隔离后返回工作岗位的 HCW 进行研究。使用侧向流动分析（LFA）设备中带强度的密度分析半定量测量 SARS-CoV-2 引起的免疫球蛋白（Ig）M 和 IgG 抗体。LFA 上每条带的平均像素强度（每英寸点数 [dpi]）被认为是抗体滴度的衡量标准。
在参与研究的 111 名 HCW 中，有 73/111（66%）的参与者检测到抗体反应。严重的 COVID 症状与年龄较大有关。男性和女性之间的 IgM 强度没有差异，但男性的 IgG 强度明显高于女性。二线 HCW 产生的 IgG 强度高于一线 HCW。重症病例的 IgG 强度较高。
对于可能感染 SARS-CoV-2 的 HCW，有必要建立常规的病毒检测程序，以避免 COVID-19 的感染和传播风险。
