Department of Biochemistry & Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, 60451, Brazil.
Department of Physic, Federal University of Ceará, Fortaleza, Ceará, 60451, Brazil.
Future Microbiol. 2022 Sep;17:1133-1146. doi: 10.2217/fmb-2022-0053. Epub 2022 Jul 26.
and are biofilm-forming drug-resistant yeasts that cause bloodstream infections that can lead to death. nystatin and itraconazole were combined with two synthetic peptides, PepGAT and PepKAA, to evaluate the synergistic effect against biofilms. Additionally, scanning electron and fluorescence microscopies were employed to understand the mechanism behind the synergistic activity. Peptides enhanced the action of drugs to inhibit the biofilm formation of and and the degradation of mature biofilms of . In combination with antifungal drugs, peptides' mechanism of action involved cell wall and membrane damage and overproduction of reactive oxygen species. Additionally, in combination, the peptides reduced the toxicity of drugs to red blood cells. These results reveal that the synthetic peptides enhanced the antibiofilm activity of drugs, in addition to reducing their toxicity. Thus, these peptides have strong potential as adjuvants and to decrease the toxicity of drugs.
和 都是形成生物膜的耐药酵母菌，可引起血流感染，导致死亡。制霉菌素和伊曲康唑与两种合成肽 PepGAT 和 PepKAA 联合使用，以评估其对 生物膜的协同作用。此外，还采用扫描电子显微镜和荧光显微镜来了解协同作用背后的机制。这些肽增强了药物的作用，抑制了 和 生物膜的形成，并降解了 的成熟生物膜。与抗真菌药物联合使用时，肽的作用机制涉及细胞壁和膜损伤以及活性氧的过度产生。此外，这些肽还降低了药物对红细胞的毒性。这些结果表明，合成肽增强了药物的抗生物膜活性，同时降低了其毒性。因此，这些肽具有作为佐剂的强大潜力，可以降低药物的毒性。
