Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, China.
Biomater Adv. 2022 Aug;139:213033. doi: 10.1016/j.bioadv.2022.213033. Epub 2022 Jul 16.
Silane adhesion layer strategy has been widely used to covalently graft biomolecules to the titanium implant surface, thereby conferring the implant bioactivity to ameliorate osseointegration. However, few researchers pay attention to the effects of silanization parameters on biocompatibility and biofunctionality of the silane adhesion layers. Accordingly, the present study successfully fabricated the silane adhesion layers with different thickness, intactness, and surface morphologies by introducing 3-aminopropyltriethoxysilane on the alkali-treated titanium surface in time-varied processing of silanization. The regulatory effects of the silane adhesion layers on angiogenesis and osteogenesis were assessed in vitro. Results showed that the prolonged silanization processing time increased the thickness and intactness of the silane adhesion layer and significantly improved its biocompatibility. Notably, the silane adhesion layer prepared after 12 h of silanization exhibited a brain-like surface morphology and benefited the adhesion and proliferation of endothelial cells (ECs) and osteoblasts (OBs). Moreover, the layer promoted angiogenesis via stimulating vascular endothelial growth factor (VEGF) secretion and nitric oxide (NO) production of ECs. Simultaneously, it improved osteogenesis by enhancing alkaline phosphatase (ALP) activity, collagen secretion, and extracellular matrix mineralization of OBs. This work systematically investigated the biocompatibility and biofunctionality of the modified silane adhesion layers, thus providing valuable references for their application in covalently grafting biomolecules on the titanium implant surface.
硅烷偶联剂粘结层策略已被广泛应用于将生物分子共价接枝到钛种植体表面，从而赋予种植体生物活性以改善骨整合。然而，很少有研究关注硅烷化参数对硅烷粘结层的生物相容性和生物功能性的影响。因此，本研究通过在碱处理的钛表面上在不同的硅烷化处理时间内引入 3-氨丙基三乙氧基硅烷，成功制备了具有不同厚度、完整性和表面形貌的硅烷粘结层。评估了硅烷粘结层对血管生成和成骨的调节作用。结果表明，延长硅烷化处理时间增加了硅烷粘结层的厚度和完整性，显著提高了其生物相容性。值得注意的是，经过 12 小时硅烷化处理制备的硅烷粘结层呈现出类似大脑的表面形貌，有利于内皮细胞（ECs）和成骨细胞（OBs）的黏附和增殖。此外，该层通过刺激血管内皮生长因子（VEGF）的分泌和内皮细胞（ECs）中一氧化氮（NO）的产生来促进血管生成。同时，它通过增强碱性磷酸酶（ALP）活性、胶原分泌和 OBs 细胞外基质矿化来改善成骨。这项工作系统地研究了改性硅烷粘结层的生物相容性和生物功能性，为其在钛种植体表面共价接枝生物分子的应用提供了有价值的参考。
