Department of Cell, Developmental and Integrative Biology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Adv Exp Med Biol. 2022;1370:369-379. doi: 10.1007/978-3-030-93337-1_35.
Taurine plays an important role in neural growth and function from early to adult life, particularly in learning and memory via BDNF action. This study tested the hypothesis that BDNF differentially potentiates entorhinal-hippocampal synaptic transmission in vivo in adult rats. In anesthetized male Sprague-Dawley rats, a stainless steel recording electrode with an attached microinjector was placed into CA1 and the dentate gyrus to record fEPSP, and a paired stainless steel electrode was inserted into entorhinal cortex for continuous paired-pulse stimulation of that brain region. In the dentate gyrus, microinjection of BDNF resulted in a gradual increase in the peak slope of the fEPSP. Following the infusion, the peak fEPSP began to rise in about 8 min, reached a maximum of 120 ± 2% (from baseline) by about 20 min, and remained near peak elevation (~115%) for more than 30 min. In contrast, the same dose of BDNF when injected into CA1 had no consistent effect on fEPSP slopes in the CA1. Further, an equimolar cytochrome C (horse heart) infusion had no significant effect on fEPSP slopes in either the dentate gyrus or CA1. The potentiation effect of BDNF in the dentate gyrus is consistent with a significant increase in power spectral density of dentate gyrus field potentials at 70-200 Hz, but not at frequencies below 70 Hz. In addition, the CA1 power spectral density was not affected by BDNF (compared to cytochrome C). These data indicate that in vivo BDNF potentiates entorhinal-hippocampal synaptic transmission in dentate gyrus, but not in CA1.
牛磺酸在从早期到成年的神经生长和功能中发挥重要作用，特别是通过 BDNF 作用促进学习和记忆。本研究检验了 BDNF 差异增强成年大鼠海马齿状回体内海马传入-海马回突触传递的假设。在麻醉雄性 Sprague-Dawley 大鼠中，将带有附加微注射器的不锈钢记录电极置于 CA1 和齿状回以记录 fEPSP，并用一对不锈钢电极插入海马前脑区以对该脑区进行连续的成对脉冲刺激。在齿状回中，BDNF 的微注射导致 fEPSP 的峰值斜率逐渐增加。输注后，峰值 fEPSP 在约 8 分钟内开始上升，约 20 分钟达到 120±2%（相对于基线）的最大值，并在 30 多分钟内保持在峰值升高附近（~115%）。相比之下，相同剂量的 BDNF 注入 CA1 对 CA1 中的 fEPSP 斜率没有一致的影响。此外，等摩尔细胞色素 C（马心）输注对齿状回或 CA1 中的 fEPSP 斜率均无明显影响。BDNF 在齿状回中的增强作用与齿状回场电位在 70-200 Hz 范围内的功率谱密度显著增加一致，但在低于 70 Hz 的频率下则不然。此外，CA1 的功率谱密度不受 BDNF（与细胞色素 C 相比）的影响。这些数据表明，BDNF 在体内增强了齿状回中的海马传入-海马回突触传递，但在 CA1 中则不然。
