Steiner C, Muller M, Baniahmad A, Renkawitz R
Nucleic Acids Res. 1987 May 26;15(10):4163-78. doi: 10.1093/nar/15.10.4163.
The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression in macrophages we performed transient DNA transfer experiments with two different types of plasmids: 5'-deletion mutants of the upstream region of the chicken lysozyme gene and different fragments from this area in front of the thymidine kinase promoter (herpes simplex virus), each placed in front of the CAT (chloramphenicol acetyl transferase) coding sequence. Two enhancers (E-2.7 kb and E-0.2 kb) were characterized. They are active in macrophages, but not in chicken fibroblasts. Furthermore a negative element (N-2.4 kb) was identified, which is active in fibroblasts and promyelocytes, but not in mature macrophages. The combined action of all three elements contributes to the observed lysozyme gene activities: no activity in fibroblasts, moderate activity in promyelocytes and high activity in mature macrophages.
鸡溶菌酶基因在巨噬细胞中组成性激活，在输卵管中受类固醇激素调控。为了研究哪些DNA元件参与其在巨噬细胞中的表达调控，我们用两种不同类型的质粒进行了瞬时DNA转染实验：鸡溶菌酶基因上游区域的5' - 缺失突变体以及来自该区域位于胸苷激酶启动子（单纯疱疹病毒）前的不同片段，每个片段都置于氯霉素乙酰转移酶（CAT）编码序列之前。鉴定出了两个增强子（E - 2.7 kb和E - 0.2 kb）。它们在巨噬细胞中具有活性，但在鸡成纤维细胞中无活性。此外，还鉴定出一个负性元件（N - 2.4 kb），它在成纤维细胞和早幼粒细胞中具有活性，但在成熟巨噬细胞中无活性。这三种元件的共同作用促成了观察到的溶菌酶基因活性：在成纤维细胞中无活性，在早幼粒细胞中有中等活性，在成熟巨噬细胞中有高活性。
