Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 639 Zhi Zao Ju Road, Shanghai, 200011, China.
Department of Ear-Nose-Throat, Eye and ENT Hospital of Fudan University, Shanghai, China.
J Orthop Surg Res. 2022 Jul 26;17(1):364. doi: 10.1186/s13018-022-03252-9.
To investigate the dynamic expression of NFAT family of periosteum in guided bone regeneration process.
The swine ribs on one side were used as the trauma group and the contralateral side as the control group. After rib segment was removed, periosteum was sutured to form a closed cavity mimicking guided bone regeneration. The periosteum and regenerated bone tissue were collected at nine time points for gene sequencing and hematoxylin-eosin staining. The expression data of each member were extracted for analysis. Expression correlations among various members were analyzed.
Staining showed the guided bone regeneration was almost completed 1 month after the operation with later stage for bone remodeling. The expression levels of each member in both groups changed greatly, especially within postoperative 1.5 months. The expression of NFATc1 and NFATC2IP in trauma group was significantly correlated with those of control group. The foldchange of each member also had large fluctuations especially within 1.5 months. In the trauma group, NFATc2 and NFATc4 were significantly upregulated, and there was a significant aggregation correlation of NFAT family expression between the various time points within one month, similar to the "pattern-block" phenomenon.
This study revealed the dynamic expression of NFAT family in guided bone regeneration, and provided a reference for the specific mechanism. The first 1.5 months is a critical period and should be paid attention to. The significant high-expression of NFATc2 and NFATc4 may role importantly in this process, which needs further research to verify it.
研究 NFAT 家族在引导骨再生过程中骨膜的动态表达。
一侧猪肋骨作为创伤组，对侧作为对照组。切除肋骨段后，将骨膜缝合形成封闭腔模拟引导骨再生。在九个时间点收集骨膜和再生骨组织进行基因测序和苏木精-伊红染色。提取各成员的表达数据进行分析。分析各成员之间的表达相关性。
染色显示术后 1 个月引导骨再生基本完成，后期进行骨重塑。两组各成员的表达水平变化较大，尤其是术后 1.5 个月内。创伤组 NFATc1 和 NFATC2IP 的表达与对照组显著相关。各成员的倍数变化也有较大波动，尤其是 1.5 个月内。在创伤组，NFATc2 和 NFATc4 明显上调，NFAT 家族在一个月内各时间点之间的表达存在明显的聚集相关性，类似于“模式块”现象。
本研究揭示了引导骨再生中 NFAT 家族的动态表达，为特定机制提供了参考。前 1.5 个月是一个关键时期，应予以重视。NFATc2 和 NFATc4 的高表达可能在该过程中起重要作用，这需要进一步研究来验证。
