Yu Bao-Fu, Li Xiao-Qing, Chen Xiao-Xue, Dai Chuan-Chang, He Jin-Guang, Wei Jiao
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
J Craniofac Surg. 2024 Sep 2. doi: 10.1097/SCS.0000000000010498.
The aim of this study was to investigate the dynamic expression of the SMAD family during guided bone regeneration for the reconstruction of cranio-maxillofacial bone defects.
A swine model of guided bone regeneration was established with one side of the rib as the trauma group and the contralateral as control group. Periosteal and regenerative tissue specimens were harvested at 9 time points in the early, middle, and late phases, and were subjected to gene sequencing and tissue staining. Expression data of each SMAD family were extracted for further analysis, in which the correlation of the expression of the respective members within and between groups and at different time points was analyzed.
The expression of individual members of the SMAD family fluctuates greatly, especially during the first month. The SMAD3 and SMAD4 genes were the most highly expressed. The foldchange value of SMAD6 was the largest and remained above 1.5 throughout the process. The dynamic expression levels of SMAD2, SMAD4, SMAD5, SMAD6, and SMAD9 showed a significant positive correlation in both groups. The expression levels of each gene showed a positive correlation with other SMAD genes. Tissue staining showed that the overall contour of the regenerated bone tissue was basically formed within the first 1 month.
The first month of guided bone regeneration is a critical period for bone regeneration and is an important period for the SMAD family to play a role. The SMAD6 may play an important role in the whole process of guided bone regeneration.
本研究旨在探讨SMAD家族在引导性骨再生修复颅颌面骨缺损过程中的动态表达情况。
建立猪引导性骨再生模型，以一侧肋骨为创伤组，对侧为对照组。在早、中、晚期9个时间点采集骨膜和再生组织标本，进行基因测序和组织染色。提取各SMAD家族的表达数据进行进一步分析，分析组内、组间及不同时间点各成员表达的相关性。
SMAD家族各成员的表达波动较大，尤其是在第1个月内。SMAD3和SMAD4基因表达最高。SMAD6的变化倍数最大，且在整个过程中均保持在1.5以上。两组中SMAD2、SMAD4、SMAD5、SMAD6和SMAD9的动态表达水平均呈显著正相关。各基因的表达水平与其他SMAD基因呈正相关。组织染色显示，再生骨组织的整体轮廓在第1个月内基本形成。
引导性骨再生的第1个月是骨再生的关键时期，也是SMAD家族发挥作用的重要时期。SMAD6可能在引导性骨再生的全过程中发挥重要作用。
