Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
J Craniofac Surg. 2024;35(5):1432-1437. doi: 10.1097/SCS.0000000000010365. Epub 2024 Jun 3.
A variety of congenital or acquired conditions can cause craniomaxillofacial bone defects, resulting in a heavy financial burden and psychological stress. Guided bone self-generation with periosteum-preserved has great potential for reconstructing large bone defects.
A swine model of guided bone regeneration with occlusive periosteum was established, the rib segment was removed, and the periosteum was sutured to form a closed regeneration chamber. Hematoxylin and eosin staining, Masson's staining, and Safranine O-Fast Green staining were done. Nine-time points were chosen for collecting the periosteum and regenerated bone tissue for gene sequencing. The expression level of each secreted frizzled-related protein (SFRP) member and the correlations among them were analyzed.
The process of bone regeneration is almost complete 1 month after surgery, and up to 1 week after surgery is an important interval for initiating the process. The expression of each SFRP family member fluctuated greatly. The highest expression level of all members ranged from 3 days to 3 months after surgery. The expression level of SFRP2 was the highest, and the difference between 2 groups was the largest. Secreted frizzled-related protein 2 and SFRP4 showed a notable positive correlation between the control and model groups. Secreted frizzled-related protein 1, SFRP2, and SFRP4 had a significant spike in fold change at 1 month postoperatively. Secreted frizzled-related protein 1 and SFRP2 had the strongest correlation.
This study revealed the dynamic expression of the SFRP family in guided bone regeneration with occlusive periosteum in a swine model, providing a possibility to advance the clinical application of bone defect repair.
多种先天性或获得性因素可导致颅颌面骨缺损，给患者带来沉重的经济负担和心理压力。带骨膜的引导骨生成技术在重建大骨缺损方面具有巨大潜力。
建立猪带骨膜闭塞性引导骨再生模型，切除肋骨段，缝合骨膜形成封闭再生室。进行苏木精-伊红染色、Masson 染色和番红 O-fast 绿染色。选择 9 个时间点采集骨膜和再生骨组织进行基因测序。分析每个分泌卷曲相关蛋白（SFRP）成员的表达水平及其相关性。
术后 1 个月骨再生过程几乎完成，术后 1 周是启动该过程的重要时间间隔。各 SFRP 家族成员的表达变化较大。所有成员的最高表达水平均在术后 3 天至 3 个月之间。所有成员中 SFRP2 的表达水平最高，两组间差异最大。SFRP2 和 SFRP4 在对照组和模型组之间呈显著正相关。SFRP1、SFRP2 和 SFRP4 在术后 1 个月时的折叠变化有明显的峰值。SFRP1 和 SFRP2 相关性最强。
本研究揭示了 SFRP 家族在猪带骨膜闭塞性引导骨再生中的动态表达，为推进骨缺损修复的临床应用提供了可能。
