Shang Yongquan, Wang Xibao, Liu Gang, Wu Xiaoyang, Wei Qinguo, Sun Guolei, Mei Xuesong, Dong Yuehuan, Sha Weilai, Zhang Honghai
College of Life Sciences, Qufu Normal University, Jingxuan West Street No. 57, Qufu 273165, China.
Animals (Basel). 2022 Jul 6;12(14):1741. doi: 10.3390/ani12141741.
The Gobiidae family occupy one of the most diverse habitat ranges of all fishes. One key reason for their successful colonization of different habitats is their ability to adapt to different energy demands. This energy requirement is related to the ability of mitochondria in cells to generate energy via oxidative phosphorylation (OXPHOS). Here, we assembled three complete mitochondrial genomes of Rhinogobius shennongensis, Rhinogobius wuyanlingensis, and Chaenogobius annularis. These mitogenomes are circular and include 13 protein-coding genes (PCGs), two rRNAs, 22 tRNAs, and one non-coding control region (CR). We used comparative mitochondrial DNA (mtDNA) genome and selection pressure analyses to explore the structure and evolutionary rates of Gobiidae mitogenomics in different environments. The CmC model showed that the ω ratios of all mtDNA PCGs were <1, and that the evolutionary rate of adenosine triphosphate 8 (atp8) was faster in Gobiidae than in other mitochondrial DNA PCGs. We also found evidence of positive selection for several sites of NADH dehydrogenase (nd) 6 and atp8 genes. Thus, divergent mechanisms appear to underlie the evolution of mtDNA PCGs, which might explain the ability of Gobiidae to adapt to diverse environments. Our study provides new insights on the adaptive evolution of Gobiidae mtDNA genome and molecular mechanisms of OXPHOS.
虾虎鱼科占据了所有鱼类中最多样化的栖息地范围之一。它们成功定殖于不同栖息地的一个关键原因是其适应不同能量需求的能力。这种能量需求与细胞中线粒体通过氧化磷酸化(OXPHOS)产生能量的能力有关。在这里,我们组装了神农栉虾虎鱼、乌岩岭栉虾虎鱼和环纹沙塘鳢的三个完整线粒体基因组。这些线粒体基因组是环状的,包括13个蛋白质编码基因(PCGs)、两个rRNA、22个tRNA和一个非编码控制区(CR)。我们使用比较线粒体DNA(mtDNA)基因组和选择压力分析来探究虾虎鱼科线粒体基因组在不同环境中的结构和进化速率。CmC模型表明,所有mtDNA PCGs的ω比率均<1,并且虾虎鱼科中三磷酸腺苷8(atp8)的进化速率比其他线粒体DNA PCGs更快。我们还发现了NADH脱氢酶(nd)6和atp8基因的几个位点存在正选择的证据。因此,不同的机制似乎是mtDNA PCGs进化的基础,这可能解释了虾虎鱼科适应多样环境的能力。我们的研究为虾虎鱼科mtDNA基因组的适应性进化和氧化磷酸化的分子机制提供了新的见解。