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Speiredonia retorta 及其他鳞翅目昆虫的线粒体基因组的结构与系统发育关系。

Organization and phylogenetic relationships of the mitochondrial genomes of Speiredonia retorta and other lepidopteran insects.

机构信息

Department of Health Inspection and Quarantine, School of Laboratory Medicine, Bengbu Medical College, Bengbu, 233004, China.

Research Center of Clinical Laboratory Science, School of Laboratory Medicine, Bengbu Medical College, 2600 Donghai Road, Bengbu, 233004, China.

出版信息

Sci Rep. 2021 Feb 3;11(1):2957. doi: 10.1038/s41598-021-82561-1.

Abstract

In this study, we analyzed the complete mitochondrial genome (mitogenome) of Speiredonia retorta, which is a pest and a member of the Lepidoptera order. In total, the S. retorta mitogenome was found to contain 15,652 base pairs encoding 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, as well as an adenine (A) + thymine (T)-rich region. These findings were consistent with the mitogenome composition of other lepidopterans, as we identified all 13 PCGs beginning at ATN codons. We also found that 11 PCGs terminated with canonical stop codons, whereas cox2 and nad4 exhibited incomplete termination codons. By analyzing the mitogenome of S. retorta using Bayesian inference (BI) and maximum likelihood (ML) models, we were able to further confirm that this species is a member of the Erebidae family.

摘要

在这项研究中,我们分析了鳞翅目害虫、卷蛾科成员旋皮夜蛾的完整线粒体基因组(mitogenome)。结果显示,旋皮夜蛾的线粒体基因组共包含 15652 个碱基对,编码 13 个蛋白质编码基因(PCGs)、22 个 tRNA、2 个 rRNA 以及一个富含腺嘌呤(A)+胸腺嘧啶(T)的区域。这些发现与其他鳞翅目昆虫的线粒体基因组组成一致,因为我们在起始密码子 ATN 处识别到了所有 13 个 PCGs。我们还发现,11 个 PCGs 以典型的终止密码子结束,而 cox2 和 nad4 则表现出不完全的终止密码子。通过对旋皮夜蛾的线粒体基因组进行贝叶斯推断(BI)和最大似然(ML)模型分析,我们进一步证实该物种属于夜蛾科。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfd8/7859238/92b22ca5c07b/41598_2021_82561_Fig1_HTML.jpg

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